Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450

Satoru Ishihara, Ken-Ichirou Morohashi, Hiroyuki Sadano, Shun-Ichiro Kawabata, Osamu Gotoh, Tsuneo Omura

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cDNA clones coding for hemoprotein H-450 were isolated from a rat liver cDNA library using anti-H-450 antibody. The molecular weight calculated from the deduced amino acid sequence comprising 547 amino acid residues was 60,085. The N-terminal sequence and a partial internal amino acid sequence of purified H-450, which were determined chemically, were both found in the amino acid sequence of H-450 deduced from the nucleotide sequence. H-450 mRNA is expressed in liver, kidney, and brain. A homology search of amino acid sequences indicated that H-450 shows no homology with cytochrome P-450, but shows significant homology with bacterial O-acetylserine (thiol)-lyases. However, H-450 has no O-acetylserine (thiol)-lyase activity.

Original languageEnglish
Pages (from-to)899-902
Number of pages4
JournalJournal of biochemistry
Issue number6
Publication statusPublished - Jan 1 1990


All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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