Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-45011β

Yasuki Nonaka, Naomichi Matsukawa, Ken ichirou Morohashi, Tsuneo Omura, Toshio Ogihara, Hiroshi Teraoka, Mitsuhiro Okamoto

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

A cDNA clone encoding cytochrome P-45011β of rat adrenal has been cloned and sequenced using a bovine P-45011β cDNA insert (pcP-450(11β)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-45011β precursor protein consisting of 499 amino acids including an extension peptide of 24 amino acids at the NH2-terminus. The cDNA contains 1247 nucleotides at the 3′-noncoding region including 51 nucleotides of poly A, but lacks the 5′-noncoding region. The deduced amino acid sequence shows 61% similarity to that of bovine P-45011β. Putative binding sites for heme and steroid are highly conserved among steroidogenic P-450s of known structure.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalFEBS Letters
Volume255
Issue number1
DOIs
Publication statusPublished - Sep 11 1989

Fingerprint

Cloning
Molecular Cloning
Cytochromes
Sequence Analysis
Rats
Complementary DNA
Amino Acids
Nucleotides
Amino Acid Sequence
Poly A
Protein Precursors
Heme
Open Reading Frames
Clone Cells
Steroids
Binding Sites
Peptides

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-45011β. / Nonaka, Yasuki; Matsukawa, Naomichi; Morohashi, Ken ichirou; Omura, Tsuneo; Ogihara, Toshio; Teraoka, Hiroshi; Okamoto, Mitsuhiro.

In: FEBS Letters, Vol. 255, No. 1, 11.09.1989, p. 21-26.

Research output: Contribution to journalArticle

Nonaka, Y, Matsukawa, N, Morohashi, KI, Omura, T, Ogihara, T, Teraoka, H & Okamoto, M 1989, 'Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-45011β', FEBS Letters, vol. 255, no. 1, pp. 21-26. https://doi.org/10.1016/0014-5793(89)81053-1
Nonaka, Yasuki ; Matsukawa, Naomichi ; Morohashi, Ken ichirou ; Omura, Tsuneo ; Ogihara, Toshio ; Teraoka, Hiroshi ; Okamoto, Mitsuhiro. / Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-45011β. In: FEBS Letters. 1989 ; Vol. 255, No. 1. pp. 21-26.
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