TY - JOUR
T1 - Molecular cloning, expression, and characterization of a novel endo-α-N-acetylgalactosaminidase from Enterococcus faecalis
AU - Goda, Hatsumi M.
AU - Ushigusa, Kota
AU - Ito, Hiromi
AU - Okino, Nozomu
AU - Narimatsu, Hisashi
AU - Ito, Makoto
N1 - Funding Information:
We thank Drs. K. Yamamoto and H. Ashida (Kyoto University, Japan) for their suggestions. We acknowledge the help of Dr. H. Wariishi (Kyushu University, Japan) with MALDI-TOF-MS. This work was supported in part by NEDO.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/10/31
Y1 - 2008/10/31
N2 - We report here the molecular cloning, expression and characterization of a novel endo-α-N-acetylgalactosaminidase, classified into the GH101 family, from Enterococcus faecalis (endo-EF). The recombinant endo-EF was found to catalyze the liberation of core1-disaccharides (Galβ1-3GalNAc) from core1-pNP (Galβ1-3GalNAcα-pNP) like other GH101 family enzymes. However, endo-EF seems to differ in specificity from the GH101 enzymes reported to date, because it was able to release trisaccharides from core2-pNP (Galβ1-3[GlcNAcβ1-6]GalNAcα-pNP) and tetrasaccharides from Gal-core2-pNP (Galβ1-3[Galβ1-3GlcNAcβ1-6]GalNAcα-pNP). Interestingly, the enzyme could transfer not only core1-disaccharides but also core2-trisaccharides to alkanols generating alkyl-oligosaccharides. Endo-EF should facilitate O-glycoprotein research.
AB - We report here the molecular cloning, expression and characterization of a novel endo-α-N-acetylgalactosaminidase, classified into the GH101 family, from Enterococcus faecalis (endo-EF). The recombinant endo-EF was found to catalyze the liberation of core1-disaccharides (Galβ1-3GalNAc) from core1-pNP (Galβ1-3GalNAcα-pNP) like other GH101 family enzymes. However, endo-EF seems to differ in specificity from the GH101 enzymes reported to date, because it was able to release trisaccharides from core2-pNP (Galβ1-3[GlcNAcβ1-6]GalNAcα-pNP) and tetrasaccharides from Gal-core2-pNP (Galβ1-3[Galβ1-3GlcNAcβ1-6]GalNAcα-pNP). Interestingly, the enzyme could transfer not only core1-disaccharides but also core2-trisaccharides to alkanols generating alkyl-oligosaccharides. Endo-EF should facilitate O-glycoprotein research.
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U2 - 10.1016/j.bbrc.2008.08.065
DO - 10.1016/j.bbrc.2008.08.065
M3 - Article
C2 - 18725192
AN - SCOPUS:51449099009
SN - 0006-291X
VL - 375
SP - 541
EP - 546
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -