Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex

Michiyo Imai, Hideo Shimada, Yukiko Okada, Yuko Matsushima-Hibiya, Tadashi Ogishima, Yuzuru Ishimura

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Using an oligonucleotide probe designed on the basis of the N-terminal amino acid sequence of purified rat aldosterone synthase cytochrome P-450 [(1989) J. Biol. Chem. 264 10935] we have isolated from rat adrenal cDNA library a 2687 base pair cDNA that encodes a protein of 500 amino acid residues. The deduced amino acid sequence contained the regions well conserved among all cytochrome P-450s sequenced to date, and also a portion (residues 25-44) which was identical to the N-terminal peptide sequence of rat aldosterone synthase cytochrome P-450. These results indicate that the cDNA encodes a precursor form of rat aldosterone synthase cytochrome P-450.

Original languageEnglish
Pages (from-to)299-302
Number of pages4
JournalFEBS Letters
Volume263
Issue number2
DOIs
Publication statusPublished - Apr 24 1990

Fingerprint

Cytochrome P-450 CYP11B2
Cloning
Adrenal Cortex
Molecular Cloning
Rats
Complementary DNA
Amino Acids
Amino Acid Sequence
Cytochrome P-450 CYP2E1
Oligonucleotide Probes
Cytochromes
Gene Library
Base Pairing
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex. / Imai, Michiyo; Shimada, Hideo; Okada, Yukiko; Matsushima-Hibiya, Yuko; Ogishima, Tadashi; Ishimura, Yuzuru.

In: FEBS Letters, Vol. 263, No. 2, 24.04.1990, p. 299-302.

Research output: Contribution to journalArticle

Imai, Michiyo ; Shimada, Hideo ; Okada, Yukiko ; Matsushima-Hibiya, Yuko ; Ogishima, Tadashi ; Ishimura, Yuzuru. / Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex. In: FEBS Letters. 1990 ; Vol. 263, No. 2. pp. 299-302.
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