Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex

Michiyo Imai, Hideo Shimada, Yukiko Okada, Yuko Matsushima-Hibiya, Tadashi Ogishima, Yuzuru Ishimura

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

Using an oligonucleotide probe designed on the basis of the N-terminal amino acid sequence of purified rat aldosterone synthase cytochrome P-450 [(1989) J. Biol. Chem. 264 10935] we have isolated from rat adrenal cDNA library a 2687 base pair cDNA that encodes a protein of 500 amino acid residues. The deduced amino acid sequence contained the regions well conserved among all cytochrome P-450s sequenced to date, and also a portion (residues 25-44) which was identical to the N-terminal peptide sequence of rat aldosterone synthase cytochrome P-450. These results indicate that the cDNA encodes a precursor form of rat aldosterone synthase cytochrome P-450.

Original languageEnglish
Pages (from-to)299-302
Number of pages4
JournalFEBS Letters
Volume263
Issue number2
DOIs
Publication statusPublished - Apr 24 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex'. Together they form a unique fingerprint.

Cite this