Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum

P. Uparanukraw, N. Morakote, T. Harnnoi, A. Dantrakool

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The advanced third-stage larvae (aL3) of Gnathostoma spinigerum contain a 24 kDa glycoprotein with diagnostic potential. Immunoscreening with the monoclonal antibody to the 24-kDa protein (mAb GN6/24) has identified a cDNA clone with an insert of 932 base pairs (bp). The insert contains a full-length gene of 732 bp encoding a protein that is 33-39% similar to matrix metalloproteinases (MMPs) of Caenorhabditis elegans and several lower and higher vertebrates. The MMP-like protein of G. spinigerum possesses the catalytic domain, but lacks the propeptide and hemopexin-like domains found in other MMPs. A signal peptide of 23 amino acids at its amino terminus indicates that it is a secretory protein, which is confirmed by Western blot analysis showing the presence of the 24 kDa protein in the excretory-secretory products of aL3.

Original languageEnglish
Pages (from-to)751-757
Number of pages7
JournalParasitology Research
Volume87
Issue number9
DOIs
Publication statusPublished - Aug 14 2001

Fingerprint

Gnathostoma
Molecular Cloning
metalloproteinases
molecular cloning
Matrix Metalloproteinases
Genes
Proteins
genes
proteins
Base Pairing
aluminum
Hemopexin
excretory-secretory products
Caenorhabditis elegans
Protein Sorting Signals
signal peptide
active sites
Larva
Vertebrates
glycoproteins

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum. / Uparanukraw, P.; Morakote, N.; Harnnoi, T.; Dantrakool, A.

In: Parasitology Research, Vol. 87, No. 9, 14.08.2001, p. 751-757.

Research output: Contribution to journalArticle

Uparanukraw, P. ; Morakote, N. ; Harnnoi, T. ; Dantrakool, A. / Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum. In: Parasitology Research. 2001 ; Vol. 87, No. 9. pp. 751-757.
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