Molecular cloning of a new member of TEKTIN family, Tektin4, located to the flagella of rat spermatozoa

Takuya Matsuyama, Yoshiko Honda, Masamichi Doiguchi, Hiroshi Iida

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Tektins are composed of a family of filament-forming proteins associated with ciliary and flagellar microtubules. A new member of the TEKTIN gene family, which was designated as rat Tektin4, was obtained by PCR technique combined with yeast two-hybrid screening. Rat Tektin4 cDNA consists of 1,341 bp encoding a 52 KDa protein of 447 amino acids. Tektin4 protein contains a Tektin domain including a nonapeptide signature sequence (RPNVELCRD), which is a prominent feature of Tektins. Its amino acid sequence showed 29%-58% identities to that of other Tektin family proteins registered in the public databases. Tektin4 gene, which was mapped to rat chromosome 10q12, is composed of six exons and spanning 5 kb. Reverse-transcriptional-PCR (RT-PCR) analysis indicated that Tektin4 was predominantly expressed in testis and its expression was upregulated during testis development. In situ hybridization analysis showed that Tektin4 mRNA was localized in round spermatids in the seminiferous tubules of the rat testis. Tektin4 protein was predominantly localized in the flagella of spermatozoa, suggesting that it might works as a flagellar component requisite for flagellar stability or sperm motility.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalMolecular Reproduction and Development
Volume72
Issue number1
DOIs
Publication statusPublished - Sep 1 2005

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Flagella
Molecular Cloning
Spermatozoa
Testis
Proteins
Polymerase Chain Reaction
Seminiferous Tubules
Spermatids
Sperm Motility
Microtubules
Genes
In Situ Hybridization
Amino Acid Sequence
Exons
Complementary DNA
Chromosomes
Yeasts
Databases
Amino Acids
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Molecular cloning of a new member of TEKTIN family, Tektin4, located to the flagella of rat spermatozoa. / Matsuyama, Takuya; Honda, Yoshiko; Doiguchi, Masamichi; Iida, Hiroshi.

In: Molecular Reproduction and Development, Vol. 72, No. 1, 01.09.2005, p. 120-128.

Research output: Contribution to journalArticle

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