Molecular cloning of a non-isopeptide-selective human endothelin receptor

Yoshihiro Ogawa; Kazuwa Nakao; Hiroshi Arai; Osamu Nakagawa; Kiminori Hosoda; Shin ichi Suga; Shigetada Nakanishi; Hiroo Imura

doi:10.1016/0006-291X(91)91806-N

Molecular cloning of a non-isopeptide-selective human endothelin receptor

Yoshihiro Ogawa, Kazuwa Nakao, Hiroshi Arai, Osamu Nakagawa, Kiminori Hosoda, Shin ichi Suga, Shigetada Nakanishi, Hiroo Imura

Research output: Contribution to journal › Article › peer-review

226 Citations (Scopus)

Abstract

We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ET_BR) from a human placenta cDNA library. The clones, different in the length of their 3′-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.

Original language	English
Pages (from-to)	248-255
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	178
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(91)91806-N
Publication status	Published - Jul 15 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(91)91806-N

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Molecular cloning of a non-isopeptide-selective human endothelin receptor. / Ogawa, Yoshihiro; Nakao, Kazuwa; Arai, Hiroshi; Nakagawa, Osamu; Hosoda, Kiminori; Suga, Shin ichi; Nakanishi, Shigetada; Imura, Hiroo.

In: Biochemical and Biophysical Research Communications, Vol. 178, No. 1, 15.07.1991, p. 248-255.

Research output: Contribution to journal › Article › peer-review

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abstract = "We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ETBR) from a human placenta cDNA library. The clones, different in the length of their 3′-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.",

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