Molecular cloning of a non-isopeptide-selective human endothelin receptor

Yoshihiro Ogawa, Kazuwa Nakao, Hiroshi Arai, Osamu Nakagawa, Kiminori Hosoda, Shin ichi Suga, Shigetada Nakanishi, Hiroo Imura

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ETBR) from a human placenta cDNA library. The clones, different in the length of their 3′-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.

Original languageEnglish
Pages (from-to)248-255
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume178
Issue number1
DOIs
Publication statusPublished - Jul 15 1991
Externally publishedYes

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this