TY - JOUR
T1 - Molecular cloning of a non-isopeptide-selective human endothelin receptor
AU - Ogawa, Yoshihiro
AU - Nakao, Kazuwa
AU - Arai, Hiroshi
AU - Nakagawa, Osamu
AU - Hosoda, Kiminori
AU - Suga, Shin ichi
AU - Nakanishi, Shigetada
AU - Imura, Hiroo
PY - 1991/7/15
Y1 - 1991/7/15
N2 - We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ETBR) from a human placenta cDNA library. The clones, different in the length of their 3′-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.
AB - We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ETBR) from a human placenta cDNA library. The clones, different in the length of their 3′-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.
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U2 - 10.1016/0006-291X(91)91806-N
DO - 10.1016/0006-291X(91)91806-N
M3 - Article
C2 - 1648908
AN - SCOPUS:0025823309
VL - 178
SP - 248
EP - 255
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -