TY - JOUR
T1 - Molecular cloning of a non-isopeptide-selective human endothelin receptor
AU - Ogawa, Yoshihiro
AU - Nakao, Kazuwa
AU - Arai, Hiroshi
AU - Nakagawa, Osamu
AU - Hosoda, Kiminori
AU - Suga, Shin ichi
AU - Nakanishi, Shigetada
AU - Imura, Hiroo
N1 - Funding Information:
Acknowledgments: We thank Ms. A. kibune and Ms. S. Takahashi for their excellent secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1990, Japan Tobacco Inc., Yamanouchi Foundation for Research on Metabolic Disorders and The Cell Science Research Foundation, 1990, Uehara Memorial Foundation, and by research grants for cardiovascular diseases (63C-2 and 2A-3) from the Japanese Ministry of Health and Welfare.
PY - 1991/7/15
Y1 - 1991/7/15
N2 - We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ETBR) from a human placenta cDNA library. The clones, different in the length of their 3′-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.
AB - We isolated several complementary DNA (cDNA) clones encoding a non-isopeptide-selective human endothelin receptor (ETBR) from a human placenta cDNA library. The clones, different in the length of their 3′-untranslated regions, encoded the same 442-amino acid protein with a transmembrane topology similar to that of other G protein-coupled receptors. The rank order of the binding of ET isopeptides (ET-1, ET-2 and ET-3) to the receptor expressed in COS-7 cells was ET-1 = ET-2 = ET-3. Northern blot analysis identified three mRNA species, 4.3 kb, 2.7 kb and 1.7 kb in size, probably generated by their use of alternative polyadenylation sites. These mRNAs were expressed in a wide variety of human tissues, at the highest level in the brain and at a significant level in cultured endothelial cells.
UR - http://www.scopus.com/inward/record.url?scp=0025823309&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025823309&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(91)91806-N
DO - 10.1016/0006-291X(91)91806-N
M3 - Article
C2 - 1648908
AN - SCOPUS:0025823309
VL - 178
SP - 248
EP - 255
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -