Molecular cloning of an activated human oncogene, homologous to v-raf, from primary stomach cancer

K. Shimizu, Y. Nakatsu, M. Sekiguchi, K. Hokamura, K. Tanaka, M. Terada, T. Sugimura

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Transfection with high molecular weight DNA from a primary stomach cancer induced foci of transformed NIH 3T3 cells, and the transformed cells were tumorigenic in nude mice. By screening with a human Alu-family probe, we isolated the human DNA sequence from the secondary transformant cells. This transforming sequence encompasses about 60 kilobase pairs and is unrelated to known human transforming genes. Examination of homologies between this sequence and retroviral oncogenes revealed that the human transforming sequence is closely related to the v-raf oncogene of murine transforming retrovirus 3611-MSV.

Original languageEnglish
Pages (from-to)5641-5645
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number17
DOIs
Publication statusPublished - 1985

All Science Journal Classification (ASJC) codes

  • General

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