Molecular cloning of an activated human oncogene, homologous to v-raf, from primary stomach cancer

K. Shimizu, Y. Nakatsu, M. Sekiguchi, K. Hokamura, K. Tanaka, M. Terada, T. Sugimura

Research output: Contribution to journalArticle

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Abstract

Transfection with high molecular weight DNA from a primary stomach cancer induced foci of transformed NIH 3T3 cells, and the transformed cells were tumorigenic in nude mice. By screening with a human Alu-family probe, we isolated the human DNA sequence from the secondary transformant cells. This transforming sequence encompasses about 60 kilobase pairs and is unrelated to known human transforming genes. Examination of homologies between this sequence and retroviral oncogenes revealed that the human transforming sequence is closely related to the v-raf oncogene of murine transforming retrovirus 3611-MSV.

Original languageEnglish
Pages (from-to)5641-5645
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number17
DOIs
Publication statusPublished - Dec 1 1985

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Molecular Cloning
Oncogenes
Stomach Neoplasms
Alu Elements
NIH 3T3 Cells
Retroviridae
Sequence Homology
Nude Mice
Transfection
Molecular Weight
DNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

Molecular cloning of an activated human oncogene, homologous to v-raf, from primary stomach cancer. / Shimizu, K.; Nakatsu, Y.; Sekiguchi, M.; Hokamura, K.; Tanaka, K.; Terada, M.; Sugimura, T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 17, 01.12.1985, p. 5641-5645.

Research output: Contribution to journalArticle

Shimizu, K. ; Nakatsu, Y. ; Sekiguchi, M. ; Hokamura, K. ; Tanaka, K. ; Terada, M. ; Sugimura, T. / Molecular cloning of an activated human oncogene, homologous to v-raf, from primary stomach cancer. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 17. pp. 5641-5645.
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