TY - JOUR
T1 - Molecular cloning of BmTUDOR-SN and analysis of its role in the RNAi pathway in the silkworm, Bombyx mori (Lepidoptera
T2 - Bombycidae)
AU - Zhu, Li
AU - Tatsuke, Tsuneyuki
AU - Li, Zhiqing
AU - Mon, Hiroaki
AU - Xu, Jian
AU - Lee, Jae Man
AU - Kusakabe, Takahiro
N1 - Funding Information:
This work was supported in part by KAKENHI no. 22248004 and 23580077 from the Japan Society for the Promotion of Science. We thank Dr. Aoki (Kyushu University Graduate School) for providing the BmN4 cell line.
PY - 2012/8
Y1 - 2012/8
N2 - Tudor-sn, a conserved nuclease, was first isolated from RNA-induced silencing complex (RISC) and was subsequently implicated in the RNA interference (RNAi) pathway in humans, flies and nematodes. However, in the silkworm, Bombyx mori L, the RNAi mechanism and the components of RISC were quite unclear. Here, we cloned the full-length cDNA of TUDOR-SN (BmTUDOR-SN) from the silkworm. Phylogenetic analysis revealed that BmTudor-sn had a high homology with Tudor-sn proteins in other insects. Fluorescent microscopic observation indicated that the subcellular localization of enhanced green fluorescent protein fused BmTudor-sn was mainly in the cytoplasm of silkworm BmN4 cells. Knockdown of BmTUDOR-SN did not, however, affect the RNAi efficiency in BmN4 cells.
AB - Tudor-sn, a conserved nuclease, was first isolated from RNA-induced silencing complex (RISC) and was subsequently implicated in the RNA interference (RNAi) pathway in humans, flies and nematodes. However, in the silkworm, Bombyx mori L, the RNAi mechanism and the components of RISC were quite unclear. Here, we cloned the full-length cDNA of TUDOR-SN (BmTUDOR-SN) from the silkworm. Phylogenetic analysis revealed that BmTudor-sn had a high homology with Tudor-sn proteins in other insects. Fluorescent microscopic observation indicated that the subcellular localization of enhanced green fluorescent protein fused BmTudor-sn was mainly in the cytoplasm of silkworm BmN4 cells. Knockdown of BmTUDOR-SN did not, however, affect the RNAi efficiency in BmN4 cells.
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U2 - 10.1007/s13355-012-0109-7
DO - 10.1007/s13355-012-0109-7
M3 - Article
AN - SCOPUS:84865720008
VL - 47
SP - 207
EP - 215
JO - Applied Entomology and Zoology
JF - Applied Entomology and Zoology
SN - 0003-6862
IS - 3
ER -