Molecular cloning of BmTUDOR-SN and analysis of its role in the RNAi pathway in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Li Zhu, Tsuneyuki Tatsuke, Zhiqing Li, Hiroaki Mon, Jian Xu, Jae Man Lee, Takahiro Kusakabe

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Abstract

Tudor-sn, a conserved nuclease, was first isolated from RNA-induced silencing complex (RISC) and was subsequently implicated in the RNA interference (RNAi) pathway in humans, flies and nematodes. However, in the silkworm, Bombyx mori L, the RNAi mechanism and the components of RISC were quite unclear. Here, we cloned the full-length cDNA of TUDOR-SN (BmTUDOR-SN) from the silkworm. Phylogenetic analysis revealed that BmTudor-sn had a high homology with Tudor-sn proteins in other insects. Fluorescent microscopic observation indicated that the subcellular localization of enhanced green fluorescent protein fused BmTudor-sn was mainly in the cytoplasm of silkworm BmN4 cells. Knockdown of BmTUDOR-SN did not, however, affect the RNAi efficiency in BmN4 cells.

Original languageEnglish
Pages (from-to)207-215
Number of pages9
JournalApplied Entomology and Zoology
Volume47
Issue number3
DOIs
Publication statusPublished - Aug 1 2012

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All Science Journal Classification (ASJC) codes

  • Insect Science

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