Molecular cloning of cDNA for cholesterol 7α-hydroxylase from rat liver microsomes. Nucleotide sequence and expression

Mitsuhide Noshiro, Masazumi Nishimoto, Ken ichirou Morohashi, Kyuichiro Okuda

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

A complete cDNA clone encoding cholesterol 7α-hydroxylase was isolated from a rat liver cDNA library by the use of specific antibodies to the enzyme. The isolated cDNA clone was 3.6 kbp long and contained a 1509-bp open reading frame encoding 503 amino acid residues (Mr = 56 880). The identity of the cDNA was confirmed by expression of cholesterol 7α-hydroxylase activity and the immunoreactive protein in COS cells transfected with pSVL expression vector carrying the cDNA insert. The primary structure of cholesterol 7α-hydroxylase deduced from the nucleotide sequence of the cDNA indicated that the enzyme constitutes a novel P-450 family. Cholesterol 7α-hydroxylase; Cytochrome P-450; cDNA cloning; (COS cell).

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalFEBS Letters
Volume257
Issue number1
DOIs
Publication statusPublished - Oct 23 1989

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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