Molecular cloning of cDNAs encoding ribonuclease-related proteins in nicotiana glutinosa leaves, as induced in response to wounding or to TMV-infection

Naomi Kurata, Tohru Kariu, Shin Kawano, Makoto Kimura

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We earlier isolated a cDNA clone (NGR1) encoding a wound-inducible ribonuclease (RNase NW) from leaves of Nicotiana glutinosa [Kariu et al. Biosci. Biotechnol. Biochem., 62, 1144–1151 (1998)]. In this study, two distinct cDNA clones, NGR2 and NGR3, encoding proteins with a ribonuclease-related sequence in the N. glutinosa leaves, were amplified and sequenced. The nucleotide sequences of NGR2 and NGR3 consist of 1244 bp and 1069 bp, and have open reading frames encoding 277 (RNase NGR2) and 236 (RNase NGR3) amino acid residues, respectively. The deduced amino acid sequences of the putative RNases NGR2 and NGR3 showed 33% and 58% amino acid sequence identity, respectively, with that of RNase NW and 32% identity with each other. Sequence comparison showed that NGR2 is similar to RNase RNS2 (61%) from Arabidopsis thaliana, while NGR3 is related to RNase LX (84%) from tomato (Lycopersicon esculentum). RNA gel blot analysis showed that the RNase NGR2 gene is constitutively expressed to measurable levels; it is not increased by either wounding or TMV infection. In contrast, the expression of the NGR3 gene is induced after 48 h upon TMV infection.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number2
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Cloning
Molecular Cloning
Ribonucleases
Tobacco
Complementary DNA
Infection
Proteins
Lycopersicon esculentum
Amino Acids
Amino Acid Sequence
Clone Cells
Genes
Arabidopsis
Open Reading Frames
Nucleotides
Gels
RNA
Gene Expression
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Molecular cloning of cDNAs encoding ribonuclease-related proteins in nicotiana glutinosa leaves, as induced in response to wounding or to TMV-infection. / Kurata, Naomi; Kariu, Tohru; Kawano, Shin; Kimura, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 2, 01.01.2002, p. 391-397.

Research output: Contribution to journalArticle

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