TY - JOUR
T1 - Molecular cloning of DAX1 and SHP cDNAs and their expression patterns in the Nile tilapia, Oreochromis niloticus
AU - Wang, De Shou
AU - Kobayashi, Tohru
AU - Senthilkumaran, Balasubramanian
AU - Sakai, Fumie
AU - Chery Sudhakumari, Cheni
AU - Suzuki, Taiga
AU - Yoshikuni, Michiyasu
AU - Matsuda, Masaru
AU - Morohashi, Ken ichirou
AU - Nagahama, Yoshitaka
N1 - Funding Information:
This work was supported in part by Grant-in-Aid for Research from CREST, JST (Japan Science and Technology Corporation), the Ministry of Education, Science, Sport and Culture of Japan, and the Ministry of Agriculture, Forestry and Fisheries (Bio Design Program). D.S.W. and C.C.S. are grateful to Japan Society for Promotion of Science for Young Scientist fellowships.
PY - 2002
Y1 - 2002
N2 - Piscine DAX1 and SHP cDNAs with an open reading frame encoding 296 and 258 amino acid residues, respectively, as well as SHP partial gene fragment, were cloned from Nile tilapia. Phylogenetic analyses of DAX1s, SHPs, and homologous EST fragments indicate that DAX1 and SHP are conserved in gene structure and are present throughout vertebrates. A single band of ∼1.4 kb for DAX1 and of ∼1.2 kb for SHP was detected in the Northern blot analysis. Tissue distribution analysis by RT-PCR showed that fish DAX1 and SHP mRNAs are widely expressed in adult tissues, with the most abundant expression in gonads and liver, respectively. DAX1 and SHP were also detected in gonads of both sexes at 5-90 days after hatching (dah). However, the expression of DAX1 is weak at 5 and 10 dah and then significantly up-regulated between 10 and 15 dah, whereas the expression of SHP is moderate and consistent during the ontogeny.
AB - Piscine DAX1 and SHP cDNAs with an open reading frame encoding 296 and 258 amino acid residues, respectively, as well as SHP partial gene fragment, were cloned from Nile tilapia. Phylogenetic analyses of DAX1s, SHPs, and homologous EST fragments indicate that DAX1 and SHP are conserved in gene structure and are present throughout vertebrates. A single band of ∼1.4 kb for DAX1 and of ∼1.2 kb for SHP was detected in the Northern blot analysis. Tissue distribution analysis by RT-PCR showed that fish DAX1 and SHP mRNAs are widely expressed in adult tissues, with the most abundant expression in gonads and liver, respectively. DAX1 and SHP were also detected in gonads of both sexes at 5-90 days after hatching (dah). However, the expression of DAX1 is weak at 5 and 10 dah and then significantly up-regulated between 10 and 15 dah, whereas the expression of SHP is moderate and consistent during the ontogeny.
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U2 - 10.1016/S0006-291X(02)02252-0
DO - 10.1016/S0006-291X(02)02252-0
M3 - Article
C2 - 12270141
AN - SCOPUS:0036383066
SN - 0006-291X
VL - 297
SP - 632
EP - 640
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -