Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA

Karla J. Matteson, Bon Chu Chung, Walter L. Miller

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.

Original languageEnglish
Pages (from-to)264-270
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume120
Issue number1
DOIs
Publication statusPublished - Apr 16 1984

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Cloning
Molecular Cloning
Complementary DNA
Messenger RNA
Clone Cells
Centrifugation
Tailings
Immunoprecipitation
Plasmids
Cholesterol
Rabbits
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA. / Matteson, Karla J.; Chung, Bon Chu; Miller, Walter L.

In: Biochemical and Biophysical Research Communications, Vol. 120, No. 1, 16.04.1984, p. 264-270.

Research output: Contribution to journalArticle

Matteson, Karla J. ; Chung, Bon Chu ; Miller, Walter L. / Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 120, No. 1. pp. 264-270.
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