Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA

Karla J. Matteson, Bon chu Chung, Walter L. Miller

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.

Original languageEnglish
Pages (from-to)264-270
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume120
Issue number1
DOIs
Publication statusPublished - Apr 16 1984

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Molecular cloning of DNA complementary to bovine adrenal P450<sub>scc</sub> mRNA'. Together they form a unique fingerprint.

  • Cite this