Molecular cloning of mouse choline kinase and choline/ethanolamine kinase

Their sequence comparison to the respective rat homologs

Chieko Aoyama, Kinichi Nakashima, Kozo Ishidate

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S. Yamashita, J. Biol. Chem. 267 (1992) 10156-10162] were isolated from a 17-day post coitum mouse embryo cDNA library and their sequences were compared with the two murine species, respectively. The nucleotide sequence homology (within the coding sequence) between mouse and rat 50-kDa choline kinases (96.0%) was considerably higher than that between their 42-kDa choline/ethanolamine kinases (92.4%). Northern blot and RT-PCR studies on several rat tissues demonstrated that both isozymes are expressed ubiquitously with the highest level in testis. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume1393
Issue number1
DOIs
Publication statusPublished - Jul 31 1998
Externally publishedYes

Fingerprint

Choline Kinase
Cloning
Molecular Cloning
Choline
Rats
Gene Library
Isoenzymes
Nucleotides
Sequence Homology
Complementary DNA
Northern Blotting
Tissue
Testis
Embryonic Structures
Polymerase Chain Reaction
ethanolamine kinase

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Biophysics

Cite this

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abstract = "Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S. Yamashita, J. Biol. Chem. 267 (1992) 10156-10162] were isolated from a 17-day post coitum mouse embryo cDNA library and their sequences were compared with the two murine species, respectively. The nucleotide sequence homology (within the coding sequence) between mouse and rat 50-kDa choline kinases (96.0{\%}) was considerably higher than that between their 42-kDa choline/ethanolamine kinases (92.4{\%}). Northern blot and RT-PCR studies on several rat tissues demonstrated that both isozymes are expressed ubiquitously with the highest level in testis. Copyright (C) 1998 Elsevier Science B.V.",
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