Molecular cloning of rat leptin receptor isoform complementary DNAs-identification of a missense mutation in Zucker fatty (fa/fa) rats

Kazuhiko Takaya, Yoshihiro Ogawa, Naohi Isse, Taku Okazaki, Noriko Satoh, Hiroaki Masuzaki, Kiyoshi Mori, Naohisa Tamura, Kiminori Hosoda, Kazuwa Nakao

Research output: Contribution to journalArticle

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Abstract

We cloned the full-length rat leptin receptor (Ob-R) isoform complementary DNAs (cDNAs) and examined the gene expression in rats. We also identified a mutation in Ob-R in Zucker fatty (fa/fa) rats. Three alternatively spliced isoforms (Ob-Ra, Ob-Rb, and Ob-Re) have been identified, which are closely related to the gp130 signal-transduction component of class I cytokine receptors. Rat Ob-Ra and Ob-Rb were single transmembrane proteins, which differ in the C-terminal amino acid sequences. On the other hand, Ob-Re had no transmembrane domain and was a soluble form of the receptor. Reverse transcription-polymerase chain reaction analysis revealed that Ob-R isoform messenger RNAs (mRNAs) are expressed in a wide variety of rat tissues in tissue-specific manners. A missense mutation (an A to C conversion at nucleotide position 806) was found in the extracellular domain of all the isoforms in Zucker fatty (fa/fa) rats, which resulted in an amino acid change from Gin to Pro at + 269 (the Gln269Pro mutation). These Ob-R isoform mRNAs were present in the brain from Zucker fatty (fa/fa)) rats at comparable amounts to those in their lean littermates. The present study provides new insight into the molecular mechanisms for Ob-R.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume225
Issue number1
DOIs
Publication statusPublished - Aug 5 1996

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Leptin Receptors
Cloning
Molecular Cloning
Missense Mutation
Rats
Protein Isoforms
Complementary DNA
RNA Isoforms
Tissue
Amino Acids
Signal transduction
Mutation
Cytokine Receptors
Polymerase chain reaction
Transcription
Gene expression
Reverse Transcription
Amino Acid Sequence
Signal Transduction
Brain

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular cloning of rat leptin receptor isoform complementary DNAs-identification of a missense mutation in Zucker fatty (fa/fa) rats. / Takaya, Kazuhiko; Ogawa, Yoshihiro; Isse, Naohi; Okazaki, Taku; Satoh, Noriko; Masuzaki, Hiroaki; Mori, Kiyoshi; Tamura, Naohisa; Hosoda, Kiminori; Nakao, Kazuwa.

In: Biochemical and Biophysical Research Communications, Vol. 225, No. 1, 05.08.1996, p. 75-83.

Research output: Contribution to journalArticle

Takaya, Kazuhiko ; Ogawa, Yoshihiro ; Isse, Naohi ; Okazaki, Taku ; Satoh, Noriko ; Masuzaki, Hiroaki ; Mori, Kiyoshi ; Tamura, Naohisa ; Hosoda, Kiminori ; Nakao, Kazuwa. / Molecular cloning of rat leptin receptor isoform complementary DNAs-identification of a missense mutation in Zucker fatty (fa/fa) rats. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 225, No. 1. pp. 75-83.
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