Molecular cloning of rat Spergen-3, a spermatogenic cell-specific gene-3, encoding a novel 75-kDa protein bearing EF-hand motifs

Hiroshi Iida, Haruhiro Yamashita, Masamichi Doiguchi, Takane Kaneko

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

By use of differential display in combination with cDNA cloning approach, we isolated a novel rat gene designated as Spergen-3, which has an open reading frame of 2055-length nucleotides encoding a protein of 685 amino acids. Spergen-3gene is composed of 15 exons and mapped on chromosome 5q36, and its mouse counterpart, which shares 85.5% identity to rat Spergen-3 at the amino acid level, is mapped on chromosome 4E1. Spergen-3 encodes a 75-kDa soluble protein bearing putative 2 EF-hand motifs, praline-repeat, and a putative nuclear localization signal. Of the 2 EF-hand motifs in Spergen-3, the second one seems to match the consensus sequence. Reverse transcription-polymerase chain reaction analysis showed that the expression of Spergen-3 is developmentally upregulated and that it is exclusively expressed in testis. In situ hybridization revealed that Spergen-3 mRNA was exclusively expressed in haploid spermatids, but its signal was weak or undetectable in spermatogonia, spermatocytes, and Sertoli cells as well as in interstitial cells. We interpreted these data as a potential role of Spergen-3, a new member of EF-hand family, in differentiation of haploid spermatids in testis.

Original languageEnglish
Pages (from-to)885-892
Number of pages8
JournalJournal of Andrology
Volume25
Issue number6
DOIs
Publication statusPublished - Jan 1 2004

Fingerprint

EF Hand Motifs
Molecular Cloning
Spermatids
Haploidy
Testis
Chromosomes
Genes
Amino Acids
Nuclear Localization Signals
Spermatogonia
Proteins
Spermatocytes
Sertoli Cells
Consensus Sequence
Open Reading Frames
Reverse Transcription
In Situ Hybridization
Organism Cloning
Exons
Nucleotides

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Endocrinology
  • Urology

Cite this

Molecular cloning of rat Spergen-3, a spermatogenic cell-specific gene-3, encoding a novel 75-kDa protein bearing EF-hand motifs. / Iida, Hiroshi; Yamashita, Haruhiro; Doiguchi, Masamichi; Kaneko, Takane.

In: Journal of Andrology, Vol. 25, No. 6, 01.01.2004, p. 885-892.

Research output: Contribution to journalArticle

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