Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio)

Miki Nakao, Satomi Hisamatsu, Makiko Nakahara, Yoko Kato, Sylvia L. Smith, Tomoki Yano

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Factor I is a novel serine protease that regulates complement activation. Here we report the complete primary structure of two isotypic factor Is isolated from the common carp (Cyprinus carpio), a pseudotetraploid teleost. A carp hepatopancreas cDNA library was screened using two RT-PCR-amplified cDNA fragments encoding part of the carp factor I-like serine protease domain. Two distinct cDNA clones, designated FI-A and FI-B, were isolated. Their deduced amino acid sequences share 75.2% identity with each other. FI-A has a typical factor I-like domain organization composed of two disulfide-linked polypeptides (H-chain and L-chain). On the other hand, FI-B contains a novel sequence of 115 amino acids inserted at the N-terminus of the H-chain. Genomic Southern hybridization suggests that FI-A and FI-B are encoded by distinct genes in the carp genome. Expression analysis by RT-PCR revealed that the major site of FI-A expression is the ovary, whereas FI-B expression is detected mainly in the hepatopancreas at a level higher than that of FI-A. The present data, taken together, suggest that carp have duplicated genes coding for factor I, and FI-B with the novel insertion plays a dominant role in the complement system. In addition, homology search of the fugu genome database using the carp FI-A and FI-B sequences identified a putative fugu factor I gene, which has an exon/intron organization different from that of the human orthologue.

Original languageEnglish
Pages (from-to)801-806
Number of pages6
JournalImmunogenetics
Volume54
Issue number11
Publication statusPublished - Feb 1 2003

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Complement Factor I
Carps
Molecular Cloning
Takifugu
Hepatopancreas
Serine Proteases
Amino Acid Sequence
Complementary DNA
Genome
Genes
Nucleic Acid Hybridization
Polymerase Chain Reaction
Complement Activation
Gene Library
Disulfides
Introns
Ovary
Exons
Clone Cells
Databases

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Nakao, M., Hisamatsu, S., Nakahara, M., Kato, Y., Smith, S. L., & Yano, T. (2003). Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio). Immunogenetics, 54(11), 801-806.

Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio). / Nakao, Miki; Hisamatsu, Satomi; Nakahara, Makiko; Kato, Yoko; Smith, Sylvia L.; Yano, Tomoki.

In: Immunogenetics, Vol. 54, No. 11, 01.02.2003, p. 801-806.

Research output: Contribution to journalArticle

Nakao, M, Hisamatsu, S, Nakahara, M, Kato, Y, Smith, SL & Yano, T 2003, 'Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio)', Immunogenetics, vol. 54, no. 11, pp. 801-806.
Nakao M, Hisamatsu S, Nakahara M, Kato Y, Smith SL, Yano T. Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio). Immunogenetics. 2003 Feb 1;54(11):801-806.
Nakao, Miki ; Hisamatsu, Satomi ; Nakahara, Makiko ; Kato, Yoko ; Smith, Sylvia L. ; Yano, Tomoki. / Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio). In: Immunogenetics. 2003 ; Vol. 54, No. 11. pp. 801-806.
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