Molecular Cloning of the Smaller Subunit (P52) of Rat Liver Mitochondrial Processing Protease

Sakae Kitada, Takuro Niidome, Takashi Nagano, Tadashi Ogishima, Akio Ito

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A cDNA encoding the smaller subunit (P52) of mitochondrial processing protease was isolated from a rat liver cDNA library, using cDNA fragment for yeast MAS1 as the probe. The deduced amino acid sequence is highly homologous to those of PEP from Neurospora crassa and MAS1 from Saccharomyces cerevisiae. After in vitro transcription and translation, the precursor peptide was imported into isolated rat liver mitochondria and processed to its mature form.

Original languageEnglish
Pages (from-to)289-293
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume190
Issue number1
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

Cloning
Molecular Cloning
Liver
Yeast
Rats
Complementary DNA
Neurospora crassa
Mitochondria
Liver Mitochondrion
Transcription
Gene Library
Saccharomyces cerevisiae
Amino Acid Sequence
Yeasts
Amino Acids
Peptides
mitochondrial processing peptidase
Peptamen
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular Cloning of the Smaller Subunit (P52) of Rat Liver Mitochondrial Processing Protease. / Kitada, Sakae; Niidome, Takuro; Nagano, Takashi; Ogishima, Tadashi; Ito, Akio.

In: Biochemical and Biophysical Research Communications, Vol. 190, No. 1, 01.01.1993, p. 289-293.

Research output: Contribution to journalArticle

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