TY - JOUR
T1 - Molecular cloning of Xenopus HGF cDNA and its expression studies in Xenopus early embryogenesis
AU - Nakamura, Hisashi
AU - Tashiro, Kosuke
AU - Nakamura, Toshikazu
AU - Shiokawa, Koichiro
N1 - Funding Information:
We thankM s. Mariko Kondo for technicaal ssistance in some of the processesd uring the isolation and se-quencingo f cDNA clones used in the presente xperi- mentsW. e thankD r. Y. Eto for kindly supplyingh uman recombinanat ctivin A. We also thank Dr. T. Sargent for generougs ift of Xenopus muscle-specifaicc tinc DNA clone. Our work was supportedi n part by Uehara MemorialF oundationa ndt heg rantsf rom theJ apanese Ministry of Education,S ciencea nd Culture.
PY - 1995/1
Y1 - 1995/1
N2 - We isolated Xenopus HGF cDNA and examined its expression pattern in Xenopus early embryos and their dissected parts. Xenopus HGF consists of 710 amino acids and contains four kringle domains and serine protease-like structure just like mammalian HGF. Northern blot analysis showed that expression of Xenopus HGF mRNA starts at the late gastrula stage and its level increases during the period of later embryogenesis. Dissection experiments revealed that Xenopus HGF mRNA is expressed in the mesoderm region, especially in the ventral mesoderm, which for the most part gives rise to mesenchymal cells. Furthermore, HGF mRNA was expressed in response to activin A and basic FGF in blastula animal cap cells. Interestingly, a stronger activity was observed with bFGF than with activin and this finding corroborates the preferential expression of HGF mRNA in the ventral mesoderm. Based on these results, we conclude that the Xenopus homologue of HGF gene is transcribed during early embryogenesis preferentially in ventral mesodermal tissues, probably in response to the signals that induce ventral mesoderm.
AB - We isolated Xenopus HGF cDNA and examined its expression pattern in Xenopus early embryos and their dissected parts. Xenopus HGF consists of 710 amino acids and contains four kringle domains and serine protease-like structure just like mammalian HGF. Northern blot analysis showed that expression of Xenopus HGF mRNA starts at the late gastrula stage and its level increases during the period of later embryogenesis. Dissection experiments revealed that Xenopus HGF mRNA is expressed in the mesoderm region, especially in the ventral mesoderm, which for the most part gives rise to mesenchymal cells. Furthermore, HGF mRNA was expressed in response to activin A and basic FGF in blastula animal cap cells. Interestingly, a stronger activity was observed with bFGF than with activin and this finding corroborates the preferential expression of HGF mRNA in the ventral mesoderm. Based on these results, we conclude that the Xenopus homologue of HGF gene is transcribed during early embryogenesis preferentially in ventral mesodermal tissues, probably in response to the signals that induce ventral mesoderm.
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U2 - 10.1016/0925-4773(94)00309-B
DO - 10.1016/0925-4773(94)00309-B
M3 - Article
C2 - 7748783
AN - SCOPUS:0028954495
SN - 0925-4773
VL - 49
SP - 123
EP - 131
JO - Cell Differentiation and Development
JF - Cell Differentiation and Development
IS - 1-2
ER -