Molecular cloning of Xenopus HGF cDNA and its expression studies in Xenopus early embryogenesis

Hisashi Nakamura, Kosuke Tashiro, Toshikazu Nakamura, Koichiro Shiokawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We isolated Xenopus HGF cDNA and examined its expression pattern in Xenopus early embryos and their dissected parts. Xenopus HGF consists of 710 amino acids and contains four kringle domains and serine protease-like structure just like mammalian HGF. Northern blot analysis showed that expression of Xenopus HGF mRNA starts at the late gastrula stage and its level increases during the period of later embryogenesis. Dissection experiments revealed that Xenopus HGF mRNA is expressed in the mesoderm region, especially in the ventral mesoderm, which for the most part gives rise to mesenchymal cells. Furthermore, HGF mRNA was expressed in response to activin A and basic FGF in blastula animal cap cells. Interestingly, a stronger activity was observed with bFGF than with activin and this finding corroborates the preferential expression of HGF mRNA in the ventral mesoderm. Based on these results, we conclude that the Xenopus homologue of HGF gene is transcribed during early embryogenesis preferentially in ventral mesodermal tissues, probably in response to the signals that induce ventral mesoderm.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalMechanisms of Development
Volume49
Issue number1-2
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

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Molecular Cloning
Xenopus
Embryonic Development
Complementary DNA
Mesoderm
Messenger RNA
Kringles
Blastula
Gastrula
Activins
Serine Proteases
Northern Blotting
Dissection
Embryonic Structures
Amino Acids
Genes

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Cite this

Molecular cloning of Xenopus HGF cDNA and its expression studies in Xenopus early embryogenesis. / Nakamura, Hisashi; Tashiro, Kosuke; Nakamura, Toshikazu; Shiokawa, Koichiro.

In: Mechanisms of Development, Vol. 49, No. 1-2, 01.01.1995, p. 123-131.

Research output: Contribution to journalArticle

Nakamura, Hisashi ; Tashiro, Kosuke ; Nakamura, Toshikazu ; Shiokawa, Koichiro. / Molecular cloning of Xenopus HGF cDNA and its expression studies in Xenopus early embryogenesis. In: Mechanisms of Development. 1995 ; Vol. 49, No. 1-2. pp. 123-131.
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