Molecular cloning, sequence analysis and expression of a cDNA encoding human type-1 angiotensin II receptor

Ryoichi Takayanagi, Keizo Ohnaka, Yoshiyuki Sakai, Ryusuke Nakao, Toshihiko Yanase, Masafumi Haji, Tadashi Inagami, Hiroaki Furuta, Deng Fu Gou, Makoto Nakamuta, Hajime Nawata

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Abstract

We isolated a cDNA encoding type-1 angiotensin II receptor from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 359 amino acid residues with a relative Mr of 41,060. The deduced amino acid sequence of the human angiotensin II (Ang II) receptor was 95.3% and 94.2% identical to those of bovine and rat type-1 Ang II receptors, respectively, and had a significant similarity with the G protein-coupled receptor. The rank order of the binding to the receptor expressed in COS-7 cells was Ang II > Ang III > Ang I. The expression of the Ang II receptor mRNA was detected in human liver, lung, adrenal and adrenocortical adenomas but not in adrenomedullary tumor, pheochromocytoma, by Northern blot analysis.

Original languageEnglish
Pages (from-to)910-916
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume183
Issue number2
DOIs
Publication statusPublished - Mar 16 1992

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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