TY - JOUR
T1 - Molecular cloning, sequence analysis and expression of a cDNA encoding human type-1 angiotensin II receptor
AU - Takayanagi, Ryoichi
AU - Ohnaka, Keizo
AU - Sakai, Yoshiyuki
AU - Nakao, Ryusuke
AU - Yanase, Toshihiko
AU - Haji, Masafumi
AU - Inagami, Tadashi
AU - Furuta, Hiroaki
AU - Gou, Deng Fu
AU - Nakamuta, Makoto
AU - Nawata, Hajime
N1 - Funding Information:
Acknowledgments : We thank Dr. D.W. Russell for providing pCMV4. This study was in part supported by a Grant-in-Aid for General Scientific Research from The Ministry of Education, Science and Culture, Japan and a Grant 1992 of Fukuoka University Hospital Clinical Research Fund.
PY - 1992/3/16
Y1 - 1992/3/16
N2 - We isolated a cDNA encoding type-1 angiotensin II receptor from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 359 amino acid residues with a relative Mr of 41,060. The deduced amino acid sequence of the human angiotensin II (Ang II) receptor was 95.3% and 94.2% identical to those of bovine and rat type-1 Ang II receptors, respectively, and had a significant similarity with the G protein-coupled receptor. The rank order of the binding to the receptor expressed in COS-7 cells was Ang II > Ang III > Ang I. The expression of the Ang II receptor mRNA was detected in human liver, lung, adrenal and adrenocortical adenomas but not in adrenomedullary tumor, pheochromocytoma, by Northern blot analysis.
AB - We isolated a cDNA encoding type-1 angiotensin II receptor from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 359 amino acid residues with a relative Mr of 41,060. The deduced amino acid sequence of the human angiotensin II (Ang II) receptor was 95.3% and 94.2% identical to those of bovine and rat type-1 Ang II receptors, respectively, and had a significant similarity with the G protein-coupled receptor. The rank order of the binding to the receptor expressed in COS-7 cells was Ang II > Ang III > Ang I. The expression of the Ang II receptor mRNA was detected in human liver, lung, adrenal and adrenocortical adenomas but not in adrenomedullary tumor, pheochromocytoma, by Northern blot analysis.
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U2 - 10.1016/0006-291X(92)90570-B
DO - 10.1016/0006-291X(92)90570-B
M3 - Article
C2 - 1550596
AN - SCOPUS:0026579883
SN - 0006-291X
VL - 183
SP - 910
EP - 916
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -