Molecular crowding effect on enzymatic reaction in a fl-microchamber to mimic crowded intracellular environment

H. Murahara, N. Kaji, M. Tokeshi, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In this work, we investigated how the size of reaction space would affect enzyme activities to understand intracellular enzyme kinetics. Two approaches were used to create pseudo-intracellular environments: a high concentration of polymers in bulk state and microfabricated chambers. Both approaches were carefully compared and we found that an enzyme activity was inhibited in 7.2 fL chamber by molecular crowding effect as in the case of highly concentrated polymer solution.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages1003-1005
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: Oct 7 2007Oct 11 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period10/7/0710/11/07

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Murahara, H., Kaji, N., Tokeshi, M., & Baba, Y. (2007). Molecular crowding effect on enzymatic reaction in a fl-microchamber to mimic crowded intracellular environment. In J-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 1003-1005). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.