Molecular design of heteroprotein assemblies providing a bionanocup as a chemical reactor

Tomomi Koshiyama, Norihiko Yokoi, Takafumi Ueno, Snuji Kanamaru, Shingo Nagano, Yoshitsugu Shiro, Fumio Arisaka, Yoshihito Watanabe

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The use of heteroprotein assemblies for providing a bionanocup to construct catalysts, sensors, and materials with novel properties, is discussed. It is used as a catalytic reaction space that consists of a heteroprotein assembly, which is a trimer of gene product 27 and gene product 5, isolated from bacteriophase T4. The bionanocup can be used for the accommodation of metal-porphyrin complexes by covalent linkage with cysteinyl thiols and its activity as an oxidation catalyst. Inductively coupled plasma (ICP) atomic-emission measurement and biocinchonianate (BCA) assay were used to observe the number of atoms in the reactor. The electron density map of derivatives shows a clear density between the male-amide moiety of FePP-MI and N7C. The proportions of Fe atoms to protein in the bionanocup were determined using the Vista-PRO (VARIAN) and bicinchonianate (BCA) methods.

Original languageEnglish
Pages (from-to)50-54
Number of pages5
JournalSmall
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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    Koshiyama, T., Yokoi, N., Ueno, T., Kanamaru, S., Nagano, S., Shiro, Y., Arisaka, F., & Watanabe, Y. (2008). Molecular design of heteroprotein assemblies providing a bionanocup as a chemical reactor. Small, 4(1), 50-54. https://doi.org/10.1002/smll.200700855