Molecular detection of circulating solid carcinoma cells in the peripheral blood: The concept of early systemic disease

Masaki Mori, Koshi Mimori, Hiroaki Ueo, Nobuya Karimine, Graham F. Barnard, Keizo Sugimachi, Tsuyoshi Akiyoshi

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Detection of the mRNA of selected genes by reverse transcriptase-polymerase chain reaction (RT-PCR) is a sensitive and powerful tool for detecting cancer cells in bone-marrow or peripheral-blood samples. In this study, we determined whether carcinoembryonic antigen (CEA) mRNA is detectable in the peripheral blood of patients with gastrointestinal or breast cancer. In addition, we studied selected patients undergoing surgical procedures to assess whether tumor manipulation during operation enhances cancer-cell dissemination. Peripheral blood from 55 patients with gastrointestinal or breast cancer and from 22 control cases was analysed for CEA mRNA using RT-PCR. For 15 selected cases undergoing curative surgery for cancer, samples were also obtained during and after surgery. The lower limit of detection was 1 to 10 CEA-positive cells diluted among 1 x 107 blood mononuclear cells. The test was positive for 20 of the 55 patients with cancer (36%). None of the 22 control samples were positive. An increase in positivity was observed with increasing stage of disease; however, even some patients with early-stage cancer showed positive results. In addition, CEA mRNA could be detected in the peripheral blood during operation in 3 of 13 patients whose pre-operative CEA mRNA in the peripheral blood had been negative. These findings suggest that, (1) RT-PCR amplification of CEA mRNA is an efficient means of detecting circulating solid cancer cells in the peripheral blood, although long-term clinical studies should be done to evaluate its usefulness; (2) not only breast cancer but also gastrointestinal cancer might be better regarded as a systemic disease even in early stages of carcinoma; and (3) surgical manipulation can provoke cancer-cell dissemination.

Original languageEnglish
Pages (from-to)739-743
Number of pages5
JournalInternational Journal of Cancer
Volume68
Issue number6
DOIs
Publication statusPublished - Dec 11 1996

Fingerprint

Carcinoembryonic Antigen
Carcinoma
Messenger RNA
Gastrointestinal Neoplasms
Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Breast Neoplasms
Bone Marrow Cells
Limit of Detection
Blood Cells
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Molecular detection of circulating solid carcinoma cells in the peripheral blood : The concept of early systemic disease. / Mori, Masaki; Mimori, Koshi; Ueo, Hiroaki; Karimine, Nobuya; Barnard, Graham F.; Sugimachi, Keizo; Akiyoshi, Tsuyoshi.

In: International Journal of Cancer, Vol. 68, No. 6, 11.12.1996, p. 739-743.

Research output: Contribution to journalArticle

Mori, Masaki ; Mimori, Koshi ; Ueo, Hiroaki ; Karimine, Nobuya ; Barnard, Graham F. ; Sugimachi, Keizo ; Akiyoshi, Tsuyoshi. / Molecular detection of circulating solid carcinoma cells in the peripheral blood : The concept of early systemic disease. In: International Journal of Cancer. 1996 ; Vol. 68, No. 6. pp. 739-743.
@article{ea80f4fba37a4e7da532686bd45b023c,
title = "Molecular detection of circulating solid carcinoma cells in the peripheral blood: The concept of early systemic disease",
abstract = "Detection of the mRNA of selected genes by reverse transcriptase-polymerase chain reaction (RT-PCR) is a sensitive and powerful tool for detecting cancer cells in bone-marrow or peripheral-blood samples. In this study, we determined whether carcinoembryonic antigen (CEA) mRNA is detectable in the peripheral blood of patients with gastrointestinal or breast cancer. In addition, we studied selected patients undergoing surgical procedures to assess whether tumor manipulation during operation enhances cancer-cell dissemination. Peripheral blood from 55 patients with gastrointestinal or breast cancer and from 22 control cases was analysed for CEA mRNA using RT-PCR. For 15 selected cases undergoing curative surgery for cancer, samples were also obtained during and after surgery. The lower limit of detection was 1 to 10 CEA-positive cells diluted among 1 x 107 blood mononuclear cells. The test was positive for 20 of the 55 patients with cancer (36{\%}). None of the 22 control samples were positive. An increase in positivity was observed with increasing stage of disease; however, even some patients with early-stage cancer showed positive results. In addition, CEA mRNA could be detected in the peripheral blood during operation in 3 of 13 patients whose pre-operative CEA mRNA in the peripheral blood had been negative. These findings suggest that, (1) RT-PCR amplification of CEA mRNA is an efficient means of detecting circulating solid cancer cells in the peripheral blood, although long-term clinical studies should be done to evaluate its usefulness; (2) not only breast cancer but also gastrointestinal cancer might be better regarded as a systemic disease even in early stages of carcinoma; and (3) surgical manipulation can provoke cancer-cell dissemination.",
author = "Masaki Mori and Koshi Mimori and Hiroaki Ueo and Nobuya Karimine and Barnard, {Graham F.} and Keizo Sugimachi and Tsuyoshi Akiyoshi",
year = "1996",
month = "12",
day = "11",
doi = "10.1002/(SICI)1097-0215(19961211)68:6<739::AID-IJC8>3.0.CO;2-4",
language = "English",
volume = "68",
pages = "739--743",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Molecular detection of circulating solid carcinoma cells in the peripheral blood

T2 - The concept of early systemic disease

AU - Mori, Masaki

AU - Mimori, Koshi

AU - Ueo, Hiroaki

AU - Karimine, Nobuya

AU - Barnard, Graham F.

AU - Sugimachi, Keizo

AU - Akiyoshi, Tsuyoshi

PY - 1996/12/11

Y1 - 1996/12/11

N2 - Detection of the mRNA of selected genes by reverse transcriptase-polymerase chain reaction (RT-PCR) is a sensitive and powerful tool for detecting cancer cells in bone-marrow or peripheral-blood samples. In this study, we determined whether carcinoembryonic antigen (CEA) mRNA is detectable in the peripheral blood of patients with gastrointestinal or breast cancer. In addition, we studied selected patients undergoing surgical procedures to assess whether tumor manipulation during operation enhances cancer-cell dissemination. Peripheral blood from 55 patients with gastrointestinal or breast cancer and from 22 control cases was analysed for CEA mRNA using RT-PCR. For 15 selected cases undergoing curative surgery for cancer, samples were also obtained during and after surgery. The lower limit of detection was 1 to 10 CEA-positive cells diluted among 1 x 107 blood mononuclear cells. The test was positive for 20 of the 55 patients with cancer (36%). None of the 22 control samples were positive. An increase in positivity was observed with increasing stage of disease; however, even some patients with early-stage cancer showed positive results. In addition, CEA mRNA could be detected in the peripheral blood during operation in 3 of 13 patients whose pre-operative CEA mRNA in the peripheral blood had been negative. These findings suggest that, (1) RT-PCR amplification of CEA mRNA is an efficient means of detecting circulating solid cancer cells in the peripheral blood, although long-term clinical studies should be done to evaluate its usefulness; (2) not only breast cancer but also gastrointestinal cancer might be better regarded as a systemic disease even in early stages of carcinoma; and (3) surgical manipulation can provoke cancer-cell dissemination.

AB - Detection of the mRNA of selected genes by reverse transcriptase-polymerase chain reaction (RT-PCR) is a sensitive and powerful tool for detecting cancer cells in bone-marrow or peripheral-blood samples. In this study, we determined whether carcinoembryonic antigen (CEA) mRNA is detectable in the peripheral blood of patients with gastrointestinal or breast cancer. In addition, we studied selected patients undergoing surgical procedures to assess whether tumor manipulation during operation enhances cancer-cell dissemination. Peripheral blood from 55 patients with gastrointestinal or breast cancer and from 22 control cases was analysed for CEA mRNA using RT-PCR. For 15 selected cases undergoing curative surgery for cancer, samples were also obtained during and after surgery. The lower limit of detection was 1 to 10 CEA-positive cells diluted among 1 x 107 blood mononuclear cells. The test was positive for 20 of the 55 patients with cancer (36%). None of the 22 control samples were positive. An increase in positivity was observed with increasing stage of disease; however, even some patients with early-stage cancer showed positive results. In addition, CEA mRNA could be detected in the peripheral blood during operation in 3 of 13 patients whose pre-operative CEA mRNA in the peripheral blood had been negative. These findings suggest that, (1) RT-PCR amplification of CEA mRNA is an efficient means of detecting circulating solid cancer cells in the peripheral blood, although long-term clinical studies should be done to evaluate its usefulness; (2) not only breast cancer but also gastrointestinal cancer might be better regarded as a systemic disease even in early stages of carcinoma; and (3) surgical manipulation can provoke cancer-cell dissemination.

UR - http://www.scopus.com/inward/record.url?scp=0030483317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030483317&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19961211)68:6<739::AID-IJC8>3.0.CO;2-4

DO - 10.1002/(SICI)1097-0215(19961211)68:6<739::AID-IJC8>3.0.CO;2-4

M3 - Article

C2 - 8980176

AN - SCOPUS:0030483317

VL - 68

SP - 739

EP - 743

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 6

ER -