Molecular dissection of the interactions among IκBα, FWD1, and Skp1 required for ubiquitin-mediated proteolysis of IκBα

Kimihiko Hattori, Shigetsugu Hatakeyama, Michiko Shirane, Masaki Matsumoto, Kei Ichi Nakayama

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The SCF complex containing Skp1, Cul1, and the F-box protein FWD1 (the mouse homologue of Drosophila Slimb and Xenopus β-TrCP) functions as the ubiquitin ligase for IκBα. FWD1 associates with Skp1 through the F-box domain and also recognizes the conserved DSGXXS motif of IκBα. The structural requirements for the interactions of FWD1 with IκBα and with Skp1 have now been investigated further. The D31A mutation (but not the G33A mutation) in the DSGXXS motif of IκBα abolished the binding of IκBα to FWD1 and its subsequent ubiquitination without affecting the phosphorylation of IκBα. The IκBα mutant D31E still exhibited binding to FWD1 and underwent ubiquitination. These results suggest that, in addition to site- specific phosphorylation at Set32 and Set36, an acidic amino acid at position 31 is required for FWD1-mediated ubiquitination of IκBα. Deletion analysis of Skp1 revealed that residues 61-143 of this protein are required for binding to FWD1. On the other hand, the highly conserved residues Pro149, Ile160, and Leu164 in the F-box domain of FWD1 were dispensable for binding to Skp1. Together, these data delineate the structural requirements for the interactions among IκBα, FWD1, and Skp1 that underlie substrate recognition by the SCF ubiquitin ligase complex.

Original languageEnglish
Pages (from-to)29641-29647
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number42
DOIs
Publication statusPublished - Oct 15 1999

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Proteolysis
Dissection
Phosphorylation
Ubiquitination
Ubiquitin
SKP Cullin F-Box Protein Ligases
F-Box Proteins
Acidic Amino Acids
Ligases
Mutation
Xenopus
Protein Binding
Drosophila
Substrates
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular dissection of the interactions among IκBα, FWD1, and Skp1 required for ubiquitin-mediated proteolysis of IκBα. / Hattori, Kimihiko; Hatakeyama, Shigetsugu; Shirane, Michiko; Matsumoto, Masaki; Nakayama, Kei Ichi.

In: Journal of Biological Chemistry, Vol. 274, No. 42, 15.10.1999, p. 29641-29647.

Research output: Contribution to journalArticle

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