Molecular evolution of a host-range gene in geminiviruses infecting asexual populations of Eupatorium makinoi

Tetsukazu Yahara, Kazuyuki Ooi, Sanae Oshita, Izumi Ishii, Masato Ikegami

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Asexual plants of Eupatorium makinoi is frequently infected with tobacco leaf curl geminivirus (TLCV). The host range of TLCV is narrow, and ORF C4 is considered to function as a host range determinant. Using this TLCV- Eupatorium system, we tested the expectation that the rate of amino acid replacements will be accelerated in ORF C4 if resistant genes of the host plants drive molecular evolution in ORF C4. ORF C4 is entirely contained within a longer ORF C1 encoding a replication protein. We analyzed 21 sequences containing ORF C4 and a part of ORF C1. While per-site number of synonymous substitutions exceeded that of replacements in ORF C1, per-site number of replacements exceeded that of synonymous substitutions in ORF C4. However, this excess of per-site replacement in ORF C4 was mostly explained by the overlap gene nature, because most synonymous substitutions in ORF C1 change amino acid of ORF C4. In conclusion, not positive but negative selection is a predominant mode characterizing molecular evolution of ORF C4.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalGenes and Genetic Systems
Volume73
Issue number3
DOIs
Publication statusPublished - Jun 1 1998

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Eupatorium
Geminiviridae
Molecular Evolution
Host Specificity
Open Reading Frames
Population
Genes
Tobacco
Amino Acids
Plant Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Molecular evolution of a host-range gene in geminiviruses infecting asexual populations of Eupatorium makinoi. / Yahara, Tetsukazu; Ooi, Kazuyuki; Oshita, Sanae; Ishii, Izumi; Ikegami, Masato.

In: Genes and Genetic Systems, Vol. 73, No. 3, 01.06.1998, p. 137-141.

Research output: Contribution to journalArticle

Yahara, Tetsukazu ; Ooi, Kazuyuki ; Oshita, Sanae ; Ishii, Izumi ; Ikegami, Masato. / Molecular evolution of a host-range gene in geminiviruses infecting asexual populations of Eupatorium makinoi. In: Genes and Genetic Systems. 1998 ; Vol. 73, No. 3. pp. 137-141.
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