Molecular evolution of amphioxus fructose-1,6 bisphosphate aldolase

Masako Kuba, Hitomi Yatsuki, Takahiro Kusakabe, Yozo Takasaki, Naruo Nikoh, Takashi Miyata, Takao Yamaguchi, Katsuji Hori

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The cDNA for amphioxus fructose-1,6-bisphosphate (FBP)-aldolase was isolated and its nucleotide sequence was determined. In the cDNA, there existed a probable open reading frame comprising 1080 bp; hence, 359 amino acid residues were deduced. The amino acid sequence indicates the deletion of 4 residues from N-terminus, in comparison with the sequence of FBP-aldolase isozymes from other sources. There was only one FBP-aldolase gene, and one enzyme species corresponding, in the amphioxus; this is the first report of the existence of a single FBP-aldolase species in animals. Enzymatic studies of both native and the recombinant FBP-aldolase suggest that the amphioxus enzyme belongs to an ancestral class I type which is not discovered among vertebrate aldolase isozymes.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume348
Issue number2
DOIs
Publication statusPublished - Dec 15 1997
Externally publishedYes

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Lancelets
Fructose-Bisphosphate Aldolase
Molecular Evolution
Isoenzymes
Complementary DNA
Amino Acids
Sequence Deletion
Enzymes
Open Reading Frames
Vertebrates
Amino Acid Sequence
Animals
Nucleotides
Genes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Molecular evolution of amphioxus fructose-1,6 bisphosphate aldolase. / Kuba, Masako; Yatsuki, Hitomi; Kusakabe, Takahiro; Takasaki, Yozo; Nikoh, Naruo; Miyata, Takashi; Yamaguchi, Takao; Hori, Katsuji.

In: Archives of Biochemistry and Biophysics, Vol. 348, No. 2, 15.12.1997, p. 329-336.

Research output: Contribution to journalArticle

Kuba, M, Yatsuki, H, Kusakabe, T, Takasaki, Y, Nikoh, N, Miyata, T, Yamaguchi, T & Hori, K 1997, 'Molecular evolution of amphioxus fructose-1,6 bisphosphate aldolase', Archives of Biochemistry and Biophysics, vol. 348, no. 2, pp. 329-336. https://doi.org/10.1006/abbi.1997.0384
Kuba, Masako ; Yatsuki, Hitomi ; Kusakabe, Takahiro ; Takasaki, Yozo ; Nikoh, Naruo ; Miyata, Takashi ; Yamaguchi, Takao ; Hori, Katsuji. / Molecular evolution of amphioxus fructose-1,6 bisphosphate aldolase. In: Archives of Biochemistry and Biophysics. 1997 ; Vol. 348, No. 2. pp. 329-336.
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