Molecular evolution of prepro-thyrotropin-releasing hormone in the chicken (Gallus gallus) and its expression in the brain

Yasuhiro Aoki, Hiroko Ono, Shinobu Yasuo, Tomohiro Masuda, Takashi Yoshimura, Shizufumi Ebihara, Masayuki Iigo, Tadashi Yanagisawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A cDNA encoding prepro-thyrotropin-relaesing hormone (ppTRH) in chicken (Callus gallus) was isolated and the sites of expression in the brain were determined. The chicken ppTRH cDNA encodes 260 amino acids, including four TRH progenitor sequences (-Lys/Arg-Arg-Gln-His-Pro-Gly-Lys/Arg-Arg-). It is interesting to note that chicken ppTRH harbors four TRH progenitor-like sequences. According to the hydropathy profile of chicken ppTRH, not only the TRH progenitor sequences but also the TRH progenitor-like sequences are localized in hydrophilic regions. The TRH progenitor-like sequences might be related to structural conservation in the evolution of ppTRH, although they cannot be processed into TRH due to the mutation of several amino acids. According to the alignment of the deduced amino-acid sequences of known vertebrate ppTRHs and the molecular phylogenetic tree we constructed, we speculate on the molecular evolution of ppTRH in vertebrates. In situ hybridization demonstrated experession of the ppTRH gene in the nucleus preopticus periventricularis, nucleus preopticus medialis, regio lateralis hypothalami, paraventricular nucleus, nucleus periventricularis hypothalami, and nucleus ventromedialis hypothalami in the chicken brain.

Original languageEnglish
Pages (from-to)686-692
Number of pages7
JournalZoological science
Volume24
Issue number7
DOIs
Publication statusPublished - Jul 1 2007

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thyrotropin-releasing hormone
Gallus gallus
thyrotropin
hormones
chickens
brain
hypothalamus
Gallus
vertebrates
paraventricular hypothalamic nucleus
amino acids
in situ hybridization
callus
amino acid sequences
mutation
phylogeny

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Molecular evolution of prepro-thyrotropin-releasing hormone in the chicken (Gallus gallus) and its expression in the brain. / Aoki, Yasuhiro; Ono, Hiroko; Yasuo, Shinobu; Masuda, Tomohiro; Yoshimura, Takashi; Ebihara, Shizufumi; Iigo, Masayuki; Yanagisawa, Tadashi.

In: Zoological science, Vol. 24, No. 7, 01.07.2007, p. 686-692.

Research output: Contribution to journalArticle

Aoki, Yasuhiro ; Ono, Hiroko ; Yasuo, Shinobu ; Masuda, Tomohiro ; Yoshimura, Takashi ; Ebihara, Shizufumi ; Iigo, Masayuki ; Yanagisawa, Tadashi. / Molecular evolution of prepro-thyrotropin-releasing hormone in the chicken (Gallus gallus) and its expression in the brain. In: Zoological science. 2007 ; Vol. 24, No. 7. pp. 686-692.
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