Molecular film assembly via layer-by-layer adsorption of oppositely charged macromolecules (linear polymer, protein and clay) and concanavalin A and glycogen

Yuri Lvov, Katsuhiko Ariga, Izumi Ichinose, Toyoki Kunitake

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Protein multilayer assemblies were prepared by two different methods: (1) alternate adsorption of oppositely charged polyions; (2) alternate adsorption with specific enzyme/substrate interaction. The formation of alternate outermost layers (opposite charge or opposite specificity) at each adsorption cycle is the key point of the procedure. Three modes of polyion film assembly were demonstrated: (a) alternation of linear polyanions and polycations; (b) insertion of charged particles in the multilayer at every other step (twelve different globular proteins were assembled alternating with Na (poly (styrenesulfonate), poly(ethyleneimine)) or Cl (poly(diallyldimethylammonium))); (c) alternation of polyions and preformed charged plates: delaminated montmorillonite clay. Specific interaction was obtained between consecutive layers of glycogen and concanavalin A. Concanavalin A possesses four binding sites for its ligand D-glucose and allows the binding of glycogen (branched poly (glucose)) layers on both sides of the protein.

Original languageEnglish
Pages (from-to)797-801
Number of pages5
JournalThin Solid Films
Volume284-285
DOIs
Publication statusPublished - Sep 15 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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