Molecular imaging contrast media for visualization of liver function

Matsuoka Yoshinori, Masaharu Murata, Huzisaki Yuri, Narahara Sayoko, Shinzato Nao, Makoto Hashizume

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background and Aims: Diagnosis of liver disease has improved because of progress in imaging technology. Among the imaging methods, magnetic resonance imaging (MRI) has the advantage of a lack of radiation exposure, but the basis of the method (imaging of hydrogen atoms in water molecules) makes it hard to detect changes in tissue or the location of the diseased tissue in the liver. The aims of this study are to develop new contrast media for visualization of functional changes in the liver and to check the effectiveness of the media. Methods: We developed a new molecular imaging contrast media that targets the asialoglycoprotein receptor (ASGP-R), a membrane protein that is specific to hepatocytes. We first checked the contrast media diameter and the cytotoxicity. Next, we examined the interaction of the media with ASGP-R through observation of fluorescein isothiocyanate (FITC)-labeled molecular imaging contrast media bound to normal hepatocellular ASGP-R using confocal laser scanning microscopy. Finally, we used MRI to observe hepatocyte interactions with the molecular imaging contrast media. Results: The contrast media forms a nanoparticle of about 30 nm diameter in aqueous solution and the cytotoxicity is low. In vitro, the media has high specificity for ASGP-R in normal rat hepatocyte RLN-8 cells and this interaction was blocked by lactose (which has a similar molecular structure to that of galactose) and by an anti-ASGP-R antibody. The contrast media markedly enhanced T1-weighted images in MRI of normal rat hepatocytes compared to the signal strength for rat liver cancer cells. Conclusions: We have shown that our new contrast media for molecular imaging of hepatocytes by MRI is effective in vitro.

Original languageEnglish
Pages (from-to)708-715
Number of pages8
JournalMagnetic Resonance Imaging
Volume28
Issue number5
DOIs
Publication statusPublished - Jun 1 2010

Fingerprint

Contrast media
Molecular imaging
Molecular Imaging
Liver
Contrast Media
Asialoglycoprotein Receptor
Visualization
Imaging techniques
Hepatocytes
Magnetic resonance
Magnetic Resonance Imaging
Rats
Cytotoxicity
Tissue
Lactose
Liver Neoplasms
Molecular Structure
Fluorescein
Galactose
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Yoshinori, M., Murata, M., Yuri, H., Sayoko, N., Nao, S., & Hashizume, M. (2010). Molecular imaging contrast media for visualization of liver function. Magnetic Resonance Imaging, 28(5), 708-715. https://doi.org/10.1016/j.mri.2010.02.003

Molecular imaging contrast media for visualization of liver function. / Yoshinori, Matsuoka; Murata, Masaharu; Yuri, Huzisaki; Sayoko, Narahara; Nao, Shinzato; Hashizume, Makoto.

In: Magnetic Resonance Imaging, Vol. 28, No. 5, 01.06.2010, p. 708-715.

Research output: Contribution to journalArticle

Yoshinori, M, Murata, M, Yuri, H, Sayoko, N, Nao, S & Hashizume, M 2010, 'Molecular imaging contrast media for visualization of liver function', Magnetic Resonance Imaging, vol. 28, no. 5, pp. 708-715. https://doi.org/10.1016/j.mri.2010.02.003
Yoshinori M, Murata M, Yuri H, Sayoko N, Nao S, Hashizume M. Molecular imaging contrast media for visualization of liver function. Magnetic Resonance Imaging. 2010 Jun 1;28(5):708-715. https://doi.org/10.1016/j.mri.2010.02.003
Yoshinori, Matsuoka ; Murata, Masaharu ; Yuri, Huzisaki ; Sayoko, Narahara ; Nao, Shinzato ; Hashizume, Makoto. / Molecular imaging contrast media for visualization of liver function. In: Magnetic Resonance Imaging. 2010 ; Vol. 28, No. 5. pp. 708-715.
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