'Molecular-imprinting' of AMP utilising the polyion complex formation process as detected by a QCM system

Yasumasa Kanekiyo; Kazuhiko Inoue; Yoshiyuki Ono; Masahito Sano; Seiji Shinkai; David N. Reinhoudt

doi:10.1039/a905965b

'Molecular-imprinting' of AMP utilising the polyion complex formation process as detected by a QCM system

Yasumasa Kanekiyo, Kazuhiko Inoue, Yoshiyuki Ono, Masahito Sano, Seiji Shinkai, David N. Reinhoudt

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

The formation of a polyion complex was applied for molecular imprinting of AMP on a QCM resonator surface. The gold electrode on the resonator surface was modified with anionic thiols. Subsequently, layers of polycations and boronic acid-containing polyanions were adsorbed onto the QCM anionic surface utilising an alternating adsorption process. When the polymer adsorption was conducted in the presence of AMP, the anionic charges of the phosphate group introduced into polyanion by a boronate-cis-diol interaction altered the film growth behaviour: viz., excess polycationic units were adsorbed onto the polyanionic surface. After removal of the AMP from the surface polyion complex, a swollen gel layer with excess cationic charge is created. It was proven that this QCM system sensitively responds to AMP. The responsiveness is derived from the mass decrease in relation to the shrinking of the surface gel layer.

Original language	English
Pages (from-to)	2719-2722
Number of pages	4
Journal	Journal of the Chemical Society. Perkin Transactions 2
Issue number	12
DOIs	https://doi.org/10.1039/a905965b
Publication status	Published - Jan 1 1999
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1039/a905965b

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title = "'Molecular-imprinting' of AMP utilising the polyion complex formation process as detected by a QCM system",

abstract = "The formation of a polyion complex was applied for molecular imprinting of AMP on a QCM resonator surface. The gold electrode on the resonator surface was modified with anionic thiols. Subsequently, layers of polycations and boronic acid-containing polyanions were adsorbed onto the QCM anionic surface utilising an alternating adsorption process. When the polymer adsorption was conducted in the presence of AMP, the anionic charges of the phosphate group introduced into polyanion by a boronate-cis-diol interaction altered the film growth behaviour: viz., excess polycationic units were adsorbed onto the polyanionic surface. After removal of the AMP from the surface polyion complex, a swollen gel layer with excess cationic charge is created. It was proven that this QCM system sensitively responds to AMP. The responsiveness is derived from the mass decrease in relation to the shrinking of the surface gel layer.",

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T1 - 'Molecular-imprinting' of AMP utilising the polyion complex formation process as detected by a QCM system

AU - Kanekiyo, Yasumasa

AU - Inoue, Kazuhiko

AU - Ono, Yoshiyuki

AU - Sano, Masahito

AU - Shinkai, Seiji

AU - Reinhoudt, David N.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The formation of a polyion complex was applied for molecular imprinting of AMP on a QCM resonator surface. The gold electrode on the resonator surface was modified with anionic thiols. Subsequently, layers of polycations and boronic acid-containing polyanions were adsorbed onto the QCM anionic surface utilising an alternating adsorption process. When the polymer adsorption was conducted in the presence of AMP, the anionic charges of the phosphate group introduced into polyanion by a boronate-cis-diol interaction altered the film growth behaviour: viz., excess polycationic units were adsorbed onto the polyanionic surface. After removal of the AMP from the surface polyion complex, a swollen gel layer with excess cationic charge is created. It was proven that this QCM system sensitively responds to AMP. The responsiveness is derived from the mass decrease in relation to the shrinking of the surface gel layer.

AB - The formation of a polyion complex was applied for molecular imprinting of AMP on a QCM resonator surface. The gold electrode on the resonator surface was modified with anionic thiols. Subsequently, layers of polycations and boronic acid-containing polyanions were adsorbed onto the QCM anionic surface utilising an alternating adsorption process. When the polymer adsorption was conducted in the presence of AMP, the anionic charges of the phosphate group introduced into polyanion by a boronate-cis-diol interaction altered the film growth behaviour: viz., excess polycationic units were adsorbed onto the polyanionic surface. After removal of the AMP from the surface polyion complex, a swollen gel layer with excess cationic charge is created. It was proven that this QCM system sensitively responds to AMP. The responsiveness is derived from the mass decrease in relation to the shrinking of the surface gel layer.

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'Molecular-imprinting' of AMP utilising the polyion complex formation process as detected by a QCM system

Abstract

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