Molecular interaction of bovine kininogen and its derivatives with papain

Tatsuya Sueyoshi, Akihiro Hara, Toshio Shimada, Makoto Kimura, Takashi Morita, Hisao Kato, Sadaaki Iwanaga

Research output: Contribution to journalArticle

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Abstract

The molecular interaction of bovine kininogen and its derivatives with papain was investigated. High-molecular-weight kininogen (HMWK) or low-molecular-weight kininogen (LMWK) and inactive papain treated with N-[N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl] agmatine (E-64) formed, respectively, a complex, which was dissocia ble on sodium dodecyl sulfate polyacrylamide-gel electrophoresis (SDS-PAGE). The densitometric determination of the bands separated on SDS-PAGE and amino acid analysis of the samples extracted from the electrophoresis gel revealed that the complex between kininogen and papain is formed in a molar ratio of one to one. Moreover, analysis of the inhibition of the caseinolytic activity of papain by these kininogens indicated that HMWK, LMWK, and kinin-free derivatives obtained from both kininogens inhibit active papain with a stoichiometry of 1 1. On the other hand, the papain activity was inhibited by two kinds of cyanogen bromide fragments isolated from the heavy chain of HMWK. These two fragments with K1 values of 38 and 0.64 nM corresponded, respectively, to residue Nos. 47 to 243 and Nos. 244-360 of the HMWK heavy chain. These results suggest that in the intact HMWK and LMWK, one of the two potential reactive sites interacts with papain to form a complex and that the other reactive site becomes active only after separation of the two sites.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalJournal of biochemistry
Volume104
Issue number2
DOIs
Publication statusPublished - Jan 1 1988

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Kininogens
Papain
Molecular interactions
High Molecular Weight Kininogens
Low Molecular Weight Kininogens
Derivatives
Electrophoresis
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Catalytic Domain
Agmatine
Cyanogen Bromide
Kinins
Stoichiometry
Gels
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Sueyoshi, T., Hara, A., Shimada, T., Kimura, M., Morita, T., Kato, H., & Iwanaga, S. (1988). Molecular interaction of bovine kininogen and its derivatives with papain. Journal of biochemistry, 104(2), 200-206. https://doi.org/10.1093/oxfordjournals.jbchem.a122442

Molecular interaction of bovine kininogen and its derivatives with papain. / Sueyoshi, Tatsuya; Hara, Akihiro; Shimada, Toshio; Kimura, Makoto; Morita, Takashi; Kato, Hisao; Iwanaga, Sadaaki.

In: Journal of biochemistry, Vol. 104, No. 2, 01.01.1988, p. 200-206.

Research output: Contribution to journalArticle

Sueyoshi, T, Hara, A, Shimada, T, Kimura, M, Morita, T, Kato, H & Iwanaga, S 1988, 'Molecular interaction of bovine kininogen and its derivatives with papain', Journal of biochemistry, vol. 104, no. 2, pp. 200-206. https://doi.org/10.1093/oxfordjournals.jbchem.a122442
Sueyoshi, Tatsuya ; Hara, Akihiro ; Shimada, Toshio ; Kimura, Makoto ; Morita, Takashi ; Kato, Hisao ; Iwanaga, Sadaaki. / Molecular interaction of bovine kininogen and its derivatives with papain. In: Journal of biochemistry. 1988 ; Vol. 104, No. 2. pp. 200-206.
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