Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases

Atsumi Tsuji-Hosokawa, Kei Takasawa, Risa Nomura, Yuichi Miyakawa, Chikahiko Numakura, Atsushi Hijikata, Tsuyoshi Shirai, Yoshihiro Ogawa, Kenichi Kashimada, Tomohiro Morio

Research output: Contribution to journalArticle

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Abstract

Background: Defects of the insulin receptor gene (INSR) cause wide spectra of congenital insulin resistance. Monoallelic defects result in milder insulin-resistant diabetes mellitus with acanthosis nigricans (IRAN, type A). Whereas, leprechaunism (Donahue syndrome), the most severe condition with lethality during the infantile period is caused by biallelic defects of INSR. Materials and Methods: We detected 2 missense mutations in 2 cases of leprechaunism and IRAN, type A, and reduced mRNA expression in the leprechaunism case. We performed an in vitro analysis to confirm that the 2 missense mutations are causative. Results: The heterozygote mutations c.3436G>A (p.Gly1146Arg) and c.294C>A (p.Ser98Arg) were identified in a male patient with IRAN, type A and a female patient with leprechaunism, respectively. Gly1146Arg was previously reported in a diabetic case without precise functional analyses, and Ser98Arg is a novel mutation. Gly1146 and Ser98 are located on the tyrosine kinase domain and ligand-binding domain of INSR, respectively, and in vitro analyses (assay for insulin binding and phosphorylation) revealed that each mutation disrupted protein functions and properties. In the leprechaunism case, mutations in INSR other than Ser98Arg were not identified, and qRT-PCR analysis revealed that mRNA expression of INSR in lymphocytes was reduced in the leprechaunism case. Conclusion: Our study indicates that the 2 missense mutations of INSR, Gly1146Arg, and Ser98Arg, are responsible for insulin resistance, and, suggests that mutations not contained within INSR, but leading to decreased INSR expression should be considered for the patients who show insulin resistance without any mutations in the coding sequence of INSR.

Original languageEnglish
Pages (from-to)917-924
Number of pages8
JournalPediatric Diabetes
Volume18
Issue number8
DOIs
Publication statusPublished - Dec 1 2017
Externally publishedYes

Fingerprint

Insulin Receptor
Donohue Syndrome
Insulin Resistance
Mutation
Genes
Missense Mutation
Messenger RNA
Heterozygote
Protein-Tyrosine Kinases
Phosphorylation
Lymphocytes
Insulin
Ligands
Gene Expression
Polymerase Chain Reaction
Diabetes Mellitus, Insulin-Resistant, with Acanthosis Nigricans

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Pediatrics, Perinatology, and Child Health
  • Endocrinology, Diabetes and Metabolism

Cite this

Tsuji-Hosokawa, A., Takasawa, K., Nomura, R., Miyakawa, Y., Numakura, C., Hijikata, A., ... Morio, T. (2017). Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases. Pediatric Diabetes, 18(8), 917-924. https://doi.org/10.1111/pedi.12508

Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases. / Tsuji-Hosokawa, Atsumi; Takasawa, Kei; Nomura, Risa; Miyakawa, Yuichi; Numakura, Chikahiko; Hijikata, Atsushi; Shirai, Tsuyoshi; Ogawa, Yoshihiro; Kashimada, Kenichi; Morio, Tomohiro.

In: Pediatric Diabetes, Vol. 18, No. 8, 01.12.2017, p. 917-924.

Research output: Contribution to journalArticle

Tsuji-Hosokawa, A, Takasawa, K, Nomura, R, Miyakawa, Y, Numakura, C, Hijikata, A, Shirai, T, Ogawa, Y, Kashimada, K & Morio, T 2017, 'Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases', Pediatric Diabetes, vol. 18, no. 8, pp. 917-924. https://doi.org/10.1111/pedi.12508
Tsuji-Hosokawa A, Takasawa K, Nomura R, Miyakawa Y, Numakura C, Hijikata A et al. Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases. Pediatric Diabetes. 2017 Dec 1;18(8):917-924. https://doi.org/10.1111/pedi.12508
Tsuji-Hosokawa, Atsumi ; Takasawa, Kei ; Nomura, Risa ; Miyakawa, Yuichi ; Numakura, Chikahiko ; Hijikata, Atsushi ; Shirai, Tsuyoshi ; Ogawa, Yoshihiro ; Kashimada, Kenichi ; Morio, Tomohiro. / Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases. In: Pediatric Diabetes. 2017 ; Vol. 18, No. 8. pp. 917-924.
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AU - Numakura, Chikahiko

AU - Hijikata, Atsushi

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AU - Kashimada, Kenichi

AU - Morio, Tomohiro

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N2 - Background: Defects of the insulin receptor gene (INSR) cause wide spectra of congenital insulin resistance. Monoallelic defects result in milder insulin-resistant diabetes mellitus with acanthosis nigricans (IRAN, type A). Whereas, leprechaunism (Donahue syndrome), the most severe condition with lethality during the infantile period is caused by biallelic defects of INSR. Materials and Methods: We detected 2 missense mutations in 2 cases of leprechaunism and IRAN, type A, and reduced mRNA expression in the leprechaunism case. We performed an in vitro analysis to confirm that the 2 missense mutations are causative. Results: The heterozygote mutations c.3436G>A (p.Gly1146Arg) and c.294C>A (p.Ser98Arg) were identified in a male patient with IRAN, type A and a female patient with leprechaunism, respectively. Gly1146Arg was previously reported in a diabetic case without precise functional analyses, and Ser98Arg is a novel mutation. Gly1146 and Ser98 are located on the tyrosine kinase domain and ligand-binding domain of INSR, respectively, and in vitro analyses (assay for insulin binding and phosphorylation) revealed that each mutation disrupted protein functions and properties. In the leprechaunism case, mutations in INSR other than Ser98Arg were not identified, and qRT-PCR analysis revealed that mRNA expression of INSR in lymphocytes was reduced in the leprechaunism case. Conclusion: Our study indicates that the 2 missense mutations of INSR, Gly1146Arg, and Ser98Arg, are responsible for insulin resistance, and, suggests that mutations not contained within INSR, but leading to decreased INSR expression should be considered for the patients who show insulin resistance without any mutations in the coding sequence of INSR.

AB - Background: Defects of the insulin receptor gene (INSR) cause wide spectra of congenital insulin resistance. Monoallelic defects result in milder insulin-resistant diabetes mellitus with acanthosis nigricans (IRAN, type A). Whereas, leprechaunism (Donahue syndrome), the most severe condition with lethality during the infantile period is caused by biallelic defects of INSR. Materials and Methods: We detected 2 missense mutations in 2 cases of leprechaunism and IRAN, type A, and reduced mRNA expression in the leprechaunism case. We performed an in vitro analysis to confirm that the 2 missense mutations are causative. Results: The heterozygote mutations c.3436G>A (p.Gly1146Arg) and c.294C>A (p.Ser98Arg) were identified in a male patient with IRAN, type A and a female patient with leprechaunism, respectively. Gly1146Arg was previously reported in a diabetic case without precise functional analyses, and Ser98Arg is a novel mutation. Gly1146 and Ser98 are located on the tyrosine kinase domain and ligand-binding domain of INSR, respectively, and in vitro analyses (assay for insulin binding and phosphorylation) revealed that each mutation disrupted protein functions and properties. In the leprechaunism case, mutations in INSR other than Ser98Arg were not identified, and qRT-PCR analysis revealed that mRNA expression of INSR in lymphocytes was reduced in the leprechaunism case. Conclusion: Our study indicates that the 2 missense mutations of INSR, Gly1146Arg, and Ser98Arg, are responsible for insulin resistance, and, suggests that mutations not contained within INSR, but leading to decreased INSR expression should be considered for the patients who show insulin resistance without any mutations in the coding sequence of INSR.

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