Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants

Prapa Songjinda; Jiro Nakayama; Yumiko Kuroki; Shigemitsu Tanaka; Sanae Fukuda; Chikako Kiyohara; Tetsuro Yamamoto; Kunio Izuchi; Taro Shirakawa; Kenji Sonomoto

doi:10.1271/bbb.69.638

Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants

Prapa Songjinda, Jiro Nakayama, Yumiko Kuroki, Shigemitsu Tanaka, Sanae Fukuda, Chikako Kiyohara, Tetsuro Yamamoto, Kunio Izuchi, Taro Shirakawa, Kenji Sonomoto

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.

Original language	English
Pages (from-to)	638-641
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	69
Issue number	3
DOIs	https://doi.org/10.1271/bbb.69.638
Publication status	Published - Mar 2005

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.69.638

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title = "Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants",

abstract = "The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.",

author = "Prapa Songjinda and Jiro Nakayama and Yumiko Kuroki and Shigemitsu Tanaka and Sanae Fukuda and Chikako Kiyohara and Tetsuro Yamamoto and Kunio Izuchi and Taro Shirakawa and Kenji Sonomoto",

note = "Funding Information: We thank all the families who provided fecal samples for this study. This research was supported in part by a Research Grant for Immunology, Allergy, and Organ Transplants from the Ministry of Health and Welfare of Japan, by a grant from Danon Institute for the Promotion of Health and Nutrition, and by a grant from Takeda Science Foundation.",

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doi = "10.1271/bbb.69.638",

language = "English",

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journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants

AU - Songjinda, Prapa

AU - Nakayama, Jiro

AU - Kuroki, Yumiko

AU - Tanaka, Shigemitsu

AU - Fukuda, Sanae

AU - Kiyohara, Chikako

AU - Yamamoto, Tetsuro

AU - Izuchi, Kunio

AU - Shirakawa, Taro

AU - Sonomoto, Kenji

N1 - Funding Information: We thank all the families who provided fecal samples for this study. This research was supported in part by a Research Grant for Immunology, Allergy, and Organ Transplants from the Ministry of Health and Welfare of Japan, by a grant from Danon Institute for the Promotion of Health and Nutrition, and by a grant from Takeda Science Foundation.

PY - 2005/3

Y1 - 2005/3

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AB - The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.

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Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants

Abstract

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