Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants

Prapa Songjinda, Jiro Nakayama, Yumiko Kuroki, Shigemitsu Tanaka, Sanae Fukuda, Chikako Kiyohara, Tetsuro Yamamoto, Kunio Izuchi, Taro Shirakawa, Kenji Sonomoto

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.

Original languageEnglish
Pages (from-to)638-641
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number3
DOIs
Publication statusPublished - Mar 1 2005

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16S Ribosomal RNA
Denaturing Gradient Gel Electrophoresis
Enterococcus
Enterobacteriaceae
Ribosomal DNA
Pseudomonas
Streptococcus
Electrophoresis
rRNA Genes
Gastrointestinal Tract
Genes
Gels
Anti-Bacterial Agents
Polymerase Chain Reaction
Monitoring
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants. / Songjinda, Prapa; Nakayama, Jiro; Kuroki, Yumiko; Tanaka, Shigemitsu; Fukuda, Sanae; Kiyohara, Chikako; Yamamoto, Tetsuro; Izuchi, Kunio; Shirakawa, Taro; Sonomoto, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 3, 01.03.2005, p. 638-641.

Research output: Contribution to journalArticle

Songjinda, Prapa ; Nakayama, Jiro ; Kuroki, Yumiko ; Tanaka, Shigemitsu ; Fukuda, Sanae ; Kiyohara, Chikako ; Yamamoto, Tetsuro ; Izuchi, Kunio ; Shirakawa, Taro ; Sonomoto, Kenji. / Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 3. pp. 638-641.
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