Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

Chenchen Liu; Yoshinori Yamaguchi; Xiaoming Dou

doi:10.1142/S0217979217440945

Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

Chenchen Liu, Yoshinori Yamaguchi, Xiaoming Dou

Research output: Contribution to journal › Article › peer-review

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Abstract

In capillary polymer electrophoresis, the property of polymer sieving matrix dominates the migration behavior of DNA/RNA. We investigated the capillary electrophoresis of RNA ranging from 100 nt to 10,000 nt in polyacrylamide (PA) solutions with different molecular weights (Mw) and different concentrations. We observed that the resolution length (RSL) of RNA fragments was improved and the migration time was prolonged, when polymer concentration was increased. The resolution for small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the large ones (>3000 nt) became inseparable. In addition, we estimated the smallest resolvable nucleotide length (Ls) by the plot of RSL against RNA size.

Original language	English
Article number	1744094
Journal	International Journal of Modern Physics B
Volume	31
Issue number	16-19
DOIs	https://doi.org/10.1142/S0217979217440945
Publication status	Published - Jul 30 2017
Externally published	Yes

All Science Journal Classification (ASJC) codes

Statistical and Nonlinear Physics
Condensed Matter Physics

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10.1142/S0217979217440945

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title = "Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis",

abstract = "In capillary polymer electrophoresis, the property of polymer sieving matrix dominates the migration behavior of DNA/RNA. We investigated the capillary electrophoresis of RNA ranging from 100 nt to 10,000 nt in polyacrylamide (PA) solutions with different molecular weights (Mw) and different concentrations. We observed that the resolution length (RSL) of RNA fragments was improved and the migration time was prolonged, when polymer concentration was increased. The resolution for small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the large ones (>3000 nt) became inseparable. In addition, we estimated the smallest resolvable nucleotide length (Ls) by the plot of RSL against RNA size.",

author = "Chenchen Liu and Yoshinori Yamaguchi and Xiaoming Dou",

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T1 - Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

AU - Liu, Chenchen

AU - Yamaguchi, Yoshinori

AU - Dou, Xiaoming

PY - 2017/7/30

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N2 - In capillary polymer electrophoresis, the property of polymer sieving matrix dominates the migration behavior of DNA/RNA. We investigated the capillary electrophoresis of RNA ranging from 100 nt to 10,000 nt in polyacrylamide (PA) solutions with different molecular weights (Mw) and different concentrations. We observed that the resolution length (RSL) of RNA fragments was improved and the migration time was prolonged, when polymer concentration was increased. The resolution for small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the large ones (>3000 nt) became inseparable. In addition, we estimated the smallest resolvable nucleotide length (Ls) by the plot of RSL against RNA size.

AB - In capillary polymer electrophoresis, the property of polymer sieving matrix dominates the migration behavior of DNA/RNA. We investigated the capillary electrophoresis of RNA ranging from 100 nt to 10,000 nt in polyacrylamide (PA) solutions with different molecular weights (Mw) and different concentrations. We observed that the resolution length (RSL) of RNA fragments was improved and the migration time was prolonged, when polymer concentration was increased. The resolution for small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the large ones (>3000 nt) became inseparable. In addition, we estimated the smallest resolvable nucleotide length (Ls) by the plot of RSL against RNA size.

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Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

Abstract

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