TY - JOUR
T1 - Molecular spectroscopic imaging using a white-light laser source
AU - Kano, Hideaki
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - Coherent anti-Stokes Raman scattering (CARS) microscopy has become a powerful technique for label-free and noninvasive imaging of chemical and biological systems at the molecular level. However, most CARS studies have focused on a limited molecular species such as lipids, and have provided monochromatic images typically at the CH stretch bands. In the present study, we have extended the CARS technique from monochromatic imaging (microscopy) to multi-color imaging (microspectroscopy). We employed a multiplex CARS process in order to obtain spectral information of the CARS signal. For the broadband Stokes laser, a white-light laser source is used. The white-light laser is generated by injecting ultrashort laser pulses into a novel photonic crystal fiber (PCF). Owing to an ultrabroadband spectral profile of the white-light laser, a wide range of the vibrational resonances can be investigated. We performed label-free and multicolor molecular spectroscopic imaging of biological samples through the multiplex CARS process.
AB - Coherent anti-Stokes Raman scattering (CARS) microscopy has become a powerful technique for label-free and noninvasive imaging of chemical and biological systems at the molecular level. However, most CARS studies have focused on a limited molecular species such as lipids, and have provided monochromatic images typically at the CH stretch bands. In the present study, we have extended the CARS technique from monochromatic imaging (microscopy) to multi-color imaging (microspectroscopy). We employed a multiplex CARS process in order to obtain spectral information of the CARS signal. For the broadband Stokes laser, a white-light laser source is used. The white-light laser is generated by injecting ultrashort laser pulses into a novel photonic crystal fiber (PCF). Owing to an ultrabroadband spectral profile of the white-light laser, a wide range of the vibrational resonances can be investigated. We performed label-free and multicolor molecular spectroscopic imaging of biological samples through the multiplex CARS process.
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U2 - 10.1246/bcsj.20100004
DO - 10.1246/bcsj.20100004
M3 - Article
AN - SCOPUS:77954712930
VL - 83
SP - 735
EP - 743
JO - Bulletin of the Chemical Society of Japan
JF - Bulletin of the Chemical Society of Japan
SN - 0009-2673
IS - 7
ER -