Molecular structure of matrices solubilized with HCl from decellularized liver and the production of albumin from hepatocytes cultured on the matrices

Shintaro Nakamura, Souta Nishimura, Hiroyuki Ijima

Research output: Contribution to journalArticle

Abstract

Solubilized liver-specific extracellular matrix (ECM) as a functional culture substratum was evaluated in this study. Briefly, the various types of solubilized ECMs were prepared from decellularized liver with HCl of various concentrations. The concentration of HCl affected the molecular structure of the solubilized ECMs. On the other hand, the level of albumin production from hepatocytes cultured on the solubilized ECMs films were higher than that of type I collagen film. However, the differences of cell adhesive tendency and albumin production of hepatocytes were could not be observed among various ECMs. Therefore, liver ECM composition is effective as a hepatocyte culture substratum.

Original languageEnglish
Pages (from-to)O-194-O-195
JournalTransactions of Japanese Society for Medical and Biological Engineering
Volume52
DOIs
Publication statusPublished - Aug 17 2014

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

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