Molecular structure of ras-related small GTP-binding protein genes of rice plants and GTPase activities of gene products in Escherichia coli

Shin ichiro Kidou, Toyoaki Anai, Masaaki Umeda, Satoshi Aotsuka, Tomohiko Tsuge, Atsushi Kato, Hirofumi Uchimiya

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We isolated two rice cDNA clones (rid and ric2) encoding proteins homologous to the ras-related small GTP-binding protein. The amino acid sequences of rid and ric2 are conserved in four regions involved in GTP binding and hydrolysis which are characteristic in the ras and ras-related small GTP-binding protein genes. In addition, two consecutive cysteine residues near the carboxyl-terminal end required for membrane anchoring are also present in rid and ric2. The rid and ric2 proteins synthesized in Escherichia coli possessed GTPase activity (i.e. hydrolysis of GTP to GDP).

Original languageEnglish
Pages (from-to)282-286
Number of pages5
JournalFEBS Letters
Volume332
Issue number3
DOIs
Publication statusPublished - Oct 18 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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