Monitor of the myostatin autocrine action during differentiation of embryonic chicken myoblasts into myotubes: Effect of IGF-I

Masatoshi Kurokawa, Fuminori Sato, Shinya Aramaki, Tomoki Soh, Nobuhiko Yamauchi, Masa Aki Hattori

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Myogenesis is regulated through the proliferation and differentiation of myoblasts expressing myostatin which functions as a negative regulator by generating Smad signals. Here, we monitored the autocrine action of myostatin in quiescent chicken myoblasts transfected with the Smad-mediated promoter reporter vector to evaluate the modulation of several growth factors. During differentiation of myoblasts into myotubes, stretched and spherical types of myoblasts were observed at 12 h after induction, at which the promoter activity began to increase. Maximal promoter activity was observed at approximately 30 h. Multinucleated myotubes were markedly formed at 72 h, but the activity was very low. IGF-I, known as a positive regulator of myogenesis, increased the promoter activity, but the increase was rather small at its high concentration (100 ng/ml). IGF-I significantly increased the level of myostatin transcript in myoblasts and newly formed myotubes at 24 h, but not at 36 h. However, the cell fusion of myoblasts was not accelerated in the presence of IGF-I. Consequently, this study indicates that the autocrine action of myostatin is partially enhanced by IGF-I through increasing its expression.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalMolecular and cellular biochemistry
Volume331
Issue number1-2
DOIs
Publication statusPublished - May 25 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Monitor of the myostatin autocrine action during differentiation of embryonic chicken myoblasts into myotubes: Effect of IGF-I'. Together they form a unique fingerprint.

Cite this