Monitoring of the microbiota profile in nukadoko, a naturally fermented rice bran bed for pickling vegetables

Hiroshi Ono, Shoko Nishio, Jun Tsurii, Tetsuhiro Kawamoto, Kenji Sonomoto, Jiro Nakayama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Nukadoko is a fermented rice bran mash traditionally used for pickling vegetables in Japan. To date, the production of both homemade and commercial nukadoko depends on natural fermentation without using starter cultures. Here, we monitored chemical and microbiological changes in the initial batch fermentation of nukadoko. Nukadoko samples were prepared by spontaneous fermentation of four different brands of rice bran, and microbiome dynamics were analyzed for 2 months. In the first week, non-Lactobacillales lactic acid bacteria (LAB) species, which differed among the samples, grew proportionally to pH decrease and lactate increase. Thereafter, Lactobacillus plantarum started growing and consumed residual sugars, causing further lactate increase in nukadoko. Finally, microbial communities in all tested nukadoko samples were dominated by L.plantarum. Taken together, our results suggest that the mixture of the fast-growing LAB species and slow-growing L.plantarum may be used as a suitable starter culture to promote the initial fermentation of nukadoko.

Original languageEnglish
Pages (from-to)520-525
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume118
Issue number5
DOIs
Publication statusPublished - Nov 1 2014

Fingerprint

Pickling
Microbiota
Vegetables
Fermentation
Lactic Acid
Starters
Monitoring
Lactic acid
Bacteria
Lactobacillus plantarum
Sugars
Japan
Oryza

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Monitoring of the microbiota profile in nukadoko, a naturally fermented rice bran bed for pickling vegetables. / Ono, Hiroshi; Nishio, Shoko; Tsurii, Jun; Kawamoto, Tetsuhiro; Sonomoto, Kenji; Nakayama, Jiro.

In: Journal of Bioscience and Bioengineering, Vol. 118, No. 5, 01.11.2014, p. 520-525.

Research output: Contribution to journalArticle

Ono, Hiroshi ; Nishio, Shoko ; Tsurii, Jun ; Kawamoto, Tetsuhiro ; Sonomoto, Kenji ; Nakayama, Jiro. / Monitoring of the microbiota profile in nukadoko, a naturally fermented rice bran bed for pickling vegetables. In: Journal of Bioscience and Bioengineering. 2014 ; Vol. 118, No. 5. pp. 520-525.
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