TY - JOUR
T1 - Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system
AU - Perez, Rodney H.
AU - Himeno, Kohei
AU - Ishibashi, Naoki
AU - Masuda, Yoshimitsu
AU - Zendo, Takeshi
AU - Fujita, Koji
AU - Wilaipun, Pongtep
AU - Leelawatcharamas, Vichien
AU - Nakayama, Jiro
AU - Sonomoto, Kenji
N1 - Funding Information:
This work was partially supported by the Ministry of Education, Culture, Sports, Science and Technology ( Monbukagakusho ) Scholarship Program of Japan, Grant-in-Aid for Scientific Research by the Japan Society for the Promotion of Science (JSPS) , the JSPS-National Research Council of Thailand (NRCT) Core University Program on "Development of Thermotolerant Microbial Resources and Their Applications”, and the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University .
PY - 2012/11
Y1 - 2012/11
N2 - Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3. The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30°C) than enterocins NKR-5-3A, C and D (25°C). Furthermore, at least 2 nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins. The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E. faecium NKR-5-3 under different fermentation conditions.
AB - Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3. The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30°C) than enterocins NKR-5-3A, C and D (25°C). Furthermore, at least 2 nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins. The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E. faecium NKR-5-3 under different fermentation conditions.
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U2 - 10.1016/j.jbiosc.2012.06.003
DO - 10.1016/j.jbiosc.2012.06.003
M3 - Article
C2 - 22762972
AN - SCOPUS:84866406275
VL - 114
SP - 490
EP - 496
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -