Monitoring primary response to chilling stress in etiolated vigna radiata and V. Mungo seedlings using thermal hysteresis of water proton NMR relaxation times

Shosuke Kaku, Mari Iwaya-inoue

Research output: Contribution to journalArticle

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Abstract

Thermal hysteresis of longitudinal relaxation times (T1) of water protons in hypocotyls of etiolated Vigna radiata and V. mungo seedlings was investigated by pulse nuclear magnetic resonance (NMR) spectroscopy. Various lengths of chilling exposures during a cool-warm cycle between 20 and 0°C (below 10°C, about 4 h) for the T1 hysteresis measurement did not cause any visible injury symptoms in hypocotyls. However, the profiles of T1 hysteresis varied as a result of different chilling exposures. The sums of the T1 ratio (for detail see Introduction) reflecting T1 prolongation or shortening upon the warming process were a good quantitative index for the extent of T1 hysteresis, and the wide dispersion of this value ranging on the "minus" side (T1 prolongation upon warming) suggested the occurrence of a primary response of cells to chilling stress before obvious visible symptoms occur while the T1 ratio sums on the "plus" side (T1 shortening upon warming) corresponded to a response of serious visible injury. Therefore, the sums of the T1 ratio can be used as a non-destructive diagnostic tool for monitoring the primary event of chilling injury when lacking any visible injury symptoms. The data indicate that the critical temperature for the occurrence of primary response for chilling stress was around 7.5°C for V. radiata and 12.5°C for V. mungo.

Original languageEnglish
Pages (from-to)1063-1072
Number of pages10
JournalPlant and Cell Physiology
Volume29
Issue number6
Publication statusPublished - Sep 1 1988

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Vigna radiata
hysteresis
Seedlings
protons
Protons
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Hot Temperature
signs and symptoms (plants)
heat
Hypocotyl
seedlings
Water
monitoring
Wounds and Injuries
shortenings
hypocotyls
water
Vigna mungo
chilling injury

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Monitoring primary response to chilling stress in etiolated vigna radiata and V. Mungo seedlings using thermal hysteresis of water proton NMR relaxation times. / Kaku, Shosuke; Iwaya-inoue, Mari.

In: Plant and Cell Physiology, Vol. 29, No. 6, 01.09.1988, p. 1063-1072.

Research output: Contribution to journalArticle

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