Monitoring redox-sensitive paramagnetic contrast agent by EPRI, OMRI and MRI

Fuminori Hyodo, Ramachandran Murugesan, Ken ichiro Matsumoto, Emi Hyodo, Sankaran Subramanian, James B. Mitchell, Murali C. Krishna

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    Abstract

    A comparative study of tissue redox-status imaging using commonly used redox sensitive nitroxides has been carried out using electron paramagnetic resonance imaging (EPRI), Overhauser magnetic resonance imaging (OMRI) and conventional T1-weighted magnetic resonance imaging, MRI. Imaging studies using phantoms of different nitroxides at different concentration levels showed that EPRI and OMRI sensitivities were found to be linearly dependent on line width of nitroxides up to 2 mM, and the enhancement in MRI intensity was linear up to 5 mM. The sensitivity and resolution of EPRI and OMRI images depended significantly on the line width of the nitroxides whereas the MRI images were almost independent of EPR line width. Reduction of the paramagnetic 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (3CP) by ascorbic acid (AsA) to the diamagnetic by hydroxylamine was monitored from a sequence of temporal images, acquired using the three imaging modalities. The decay rates determined by all the three modalities were found to be similar. However the results suggest that T1-weighted MRI can monitor the redox status, in addition to providing detailed anatomical structure in a short time. Therefore, a combination of MRI with nitroxides as metabolically responsive contrast agents can be a useful technique for the in vivo imaging probing tissue redox status.

    Original languageEnglish
    Pages (from-to)105-112
    Number of pages8
    JournalJournal of Magnetic Resonance
    Volume190
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 2008

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    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Nuclear and High Energy Physics
    • Condensed Matter Physics

    Cite this

    Hyodo, F., Murugesan, R., Matsumoto, K. I., Hyodo, E., Subramanian, S., Mitchell, J. B., & Krishna, M. C. (2008). Monitoring redox-sensitive paramagnetic contrast agent by EPRI, OMRI and MRI. Journal of Magnetic Resonance, 190(1), 105-112. https://doi.org/10.1016/j.jmr.2007.10.013