Monoclonal antibodies that depress a specific subset of multiple components of the glutathione-induced response of Hydra

Masahiko Sakaguchi, Kazumitsu Hanai, Kunimasa Ohta, Masaaki Kitajima, Sachiko Matsuhashi, Katsuji Hori, Hiromichi Morita

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Reduced glutathione evokes a feeding response, the tentacle-ball formation in Hydra japonica. This response consists of at least 5 components (R1-R5). We raised 6 monoclonal antibodies (mAbs), each of which depressed a specific subset of these components, and we also examined the immunocytochemical localization of antigens with these mAbs at light microscopic level. The 2 mAbs that depressed R2 and R4 bound to the cnidocils of the desmoneme and the stenotele nematocytes; the 3 mAbs that depressed R5 bound to the apical surface adjacent to the cnidocils of the nematocytes; and the 2 mAbs that depressed R1 and R3 bound to the apical spot structures of unidentified cells in the ectoderm. Together with the specificity of the action of the mAbs on the behavioral response, the correspondence between the effects on the response and the structures visualized with these mAbs suggests that these structures include components of the receptor-effector system relevant to chemoreception.

Original languageEnglish
Pages (from-to)409-416
Number of pages8
JournalJournal of Comparative Physiology A
Volume168
Issue number4
DOIs
Publication statusPublished - Apr 1 1991

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Animal Science and Zoology
  • Behavioral Neuroscience

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