Monoclonal antibody based enzyme immunoassay for marihuana (cannabinoid) compounds

Hiroyuki Tanaka, Yuri Goto, Yukihiro Shoyama

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51 Citations (Scopus)

Abstract

MAb against Δ1-THCA was produced by fusing hybridoma with splenocytes immunized with Δ1-THCA-BSA conjugate and hypoxanthine, aminopterine, thymidine-sensitive mouse myeloma cell line, P3-X63-Ag8-653. The cross- reaction of anti-Δ1-THCA antibody against other cannabinoids was very wide, thus many cannabinoids and a spiro-compound were reactive suggesting that 2'- hydroxyl, 6'-hydroxyl or 6'-O-alkyl, 4'-alkylbenzene ring moiety is necessary for its reactivity. It became evident that this ELISA was able to be applied to the biotransformation experiments of cannabinoids in plant tissue culture system. The metabolites of Δ6-THC such as two major metabolites. 7-oxo- Δ6-THC and 7-hydroxyl-Δ6-THC were also detectable by this ELISA.

Original languageEnglish
Pages (from-to)321-342
Number of pages22
JournalJournal of Immunoassay
Volume17
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

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