TY - JOUR
T1 - Monoclonal antibody specific for Dhx9/NDHII/RHA
AU - Kotani, Manato
AU - Harada, Akihito
AU - Odawara, Jun
AU - Azuma, Masayuki
AU - Okada, Seiji
AU - Nishiyama, Yuko
AU - Nakamura, Mako
AU - Tachibana, Taro
AU - Ohkawa, Yasuyuki
PY - 2010/6/1
Y1 - 2010/6/1
N2 - Dhx9/NDHII/RHA is a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain. The DEAH protein family plays a critical role in RNA metabolism. DEAH family members function as ATP-dependent RNA helicases and regulation of transcription. In the present study, we report the establishment of a monoclonal antibody specific for Dhx9 using the rat medial iliac lymph node method. Immunoblot analysis using our antibody against Dhx9 detected full-length Dhx9. In addition, immunocytochemical staining using our antibody against Dhx9 revealed the nuclear localization of Dhx9. This monoclonal antibody against Dhx9 will allow for further detailed studies of Dhx9 expression.
AB - Dhx9/NDHII/RHA is a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain. The DEAH protein family plays a critical role in RNA metabolism. DEAH family members function as ATP-dependent RNA helicases and regulation of transcription. In the present study, we report the establishment of a monoclonal antibody specific for Dhx9 using the rat medial iliac lymph node method. Immunoblot analysis using our antibody against Dhx9 detected full-length Dhx9. In addition, immunocytochemical staining using our antibody against Dhx9 revealed the nuclear localization of Dhx9. This monoclonal antibody against Dhx9 will allow for further detailed studies of Dhx9 expression.
UR - http://www.scopus.com/inward/record.url?scp=77954186821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954186821&partnerID=8YFLogxK
U2 - 10.1089/hyb.2009.0107
DO - 10.1089/hyb.2009.0107
M3 - Article
C2 - 20569003
AN - SCOPUS:77954186821
SN - 2167-9436
VL - 29
SP - 259
EP - 261
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 3
ER -