Monoclonal antibody specific for Dhx9/NDHII/RHA

Manato Kotani, Akihito Harada, Jun Odawara, Masayuki Azuma, Seiji Okada, Yuko Nishiyama, Mako Nakamura, Taro Tachibana, Yasuyuki Ohkawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dhx9/NDHII/RHA is a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain. The DEAH protein family plays a critical role in RNA metabolism. DEAH family members function as ATP-dependent RNA helicases and regulation of transcription. In the present study, we report the establishment of a monoclonal antibody specific for Dhx9 using the rat medial iliac lymph node method. Immunoblot analysis using our antibody against Dhx9 detected full-length Dhx9. In addition, immunocytochemical staining using our antibody against Dhx9 revealed the nuclear localization of Dhx9. This monoclonal antibody against Dhx9 will allow for further detailed studies of Dhx9 expression.

Original languageEnglish
Pages (from-to)259-261
Number of pages3
JournalHybridoma
Volume29
Issue number3
DOIs
Publication statusPublished - Jun 1 2010

Fingerprint

Monoclonal Antibodies
RNA Helicases
Antibodies
Proteins
Adenosine Triphosphate
Lymph Nodes
RNA
Staining and Labeling
Double-Stranded RNA Binding Motif

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Monoclonal antibody specific for Dhx9/NDHII/RHA. / Kotani, Manato; Harada, Akihito; Odawara, Jun; Azuma, Masayuki; Okada, Seiji; Nishiyama, Yuko; Nakamura, Mako; Tachibana, Taro; Ohkawa, Yasuyuki.

In: Hybridoma, Vol. 29, No. 3, 01.06.2010, p. 259-261.

Research output: Contribution to journalArticle

Kotani, M, Harada, A, Odawara, J, Azuma, M, Okada, S, Nishiyama, Y, Nakamura, M, Tachibana, T & Ohkawa, Y 2010, 'Monoclonal antibody specific for Dhx9/NDHII/RHA', Hybridoma, vol. 29, no. 3, pp. 259-261. https://doi.org/10.1089/hyb.2009.0107
Kotani M, Harada A, Odawara J, Azuma M, Okada S, Nishiyama Y et al. Monoclonal antibody specific for Dhx9/NDHII/RHA. Hybridoma. 2010 Jun 1;29(3):259-261. https://doi.org/10.1089/hyb.2009.0107
Kotani, Manato ; Harada, Akihito ; Odawara, Jun ; Azuma, Masayuki ; Okada, Seiji ; Nishiyama, Yuko ; Nakamura, Mako ; Tachibana, Taro ; Ohkawa, Yasuyuki. / Monoclonal antibody specific for Dhx9/NDHII/RHA. In: Hybridoma. 2010 ; Vol. 29, No. 3. pp. 259-261.
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AU - Nishiyama, Yuko

AU - Nakamura, Mako

AU - Tachibana, Taro

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