Monoclonal antibody to a specific antigen from Wolinella recta ATCC 33238

Hidemi Kurihara; Fusanori Nishimura; Susumu Kokeguchi; Keijiro Kato; Thomas E. Van Dyke; Yoji Murayama

doi:10.1111/j.1574-6968.1991.tb04835.x

Monoclonal antibody to a specific antigen from Wolinella recta ATCC 33238

Hidemi Kurihara, Fusanori Nishimura, Susumu Kokeguchi, Keijiro Kato, Thomas E. Van Dyke, Yoji Murayama

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Four monoclonal antibodies (mAbs) to a specific antigen (150-kDa protein antigen) isolated from Wolinella recta ATCC 33238 by acid extraction were obtained. The four antibodies were all of the IgG1 subclass and exhibited equally high specificity for the antigen. A battery of 14 strains of oral bacteria were screened for cross-reactivity with each mAb by ELISA and Western blot analysis. Weak cross-reactivity to some strains was observed which differed depending upon the mAb. Immuno-electronmicroscopic studies were performed with a mAb which revealed that the 150-kDa antigen was localized to the cell surface of W. recta ATCC 33238.

Original language	English
Pages (from-to)	33-36
Number of pages	4
Journal	FEMS microbiology letters
Volume	82
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04835.x
Publication status	Published - Jul 15 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1991.tb04835.x

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abstract = "Four monoclonal antibodies (mAbs) to a specific antigen (150-kDa protein antigen) isolated from Wolinella recta ATCC 33238 by acid extraction were obtained. The four antibodies were all of the IgG1 subclass and exhibited equally high specificity for the antigen. A battery of 14 strains of oral bacteria were screened for cross-reactivity with each mAb by ELISA and Western blot analysis. Weak cross-reactivity to some strains was observed which differed depending upon the mAb. Immuno-electronmicroscopic studies were performed with a mAb which revealed that the 150-kDa antigen was localized to the cell surface of W. recta ATCC 33238.",

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AU - Kurihara, Hidemi

AU - Nishimura, Fusanori

AU - Kokeguchi, Susumu

AU - Kato, Keijiro

AU - Van Dyke, Thomas E.

AU - Murayama, Yoji

PY - 1991/7/15

Y1 - 1991/7/15

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AB - Four monoclonal antibodies (mAbs) to a specific antigen (150-kDa protein antigen) isolated from Wolinella recta ATCC 33238 by acid extraction were obtained. The four antibodies were all of the IgG1 subclass and exhibited equally high specificity for the antigen. A battery of 14 strains of oral bacteria were screened for cross-reactivity with each mAb by ELISA and Western blot analysis. Weak cross-reactivity to some strains was observed which differed depending upon the mAb. Immuno-electronmicroscopic studies were performed with a mAb which revealed that the 150-kDa antigen was localized to the cell surface of W. recta ATCC 33238.

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Monoclonal antibody to a specific antigen from Wolinella recta ATCC 33238

Abstract

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