Morphological analysis of mouse pulmonary acini extracted from synchrotron micro-CT images with a multiscale-based segmentation algorithm

Luosha Xiao, Toshihiro Sera, Kenichiro Koshiyama, Shigeo Wada

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A semi-automatic segmentation method for extracting the complete structure of pulmonary acinus from synchrotron micro-CT images was developed on the basis of the multiscale and hierarchical nature of lung structures. To verify the potential of the method for the statistical analysis of acinar morphology, we here measured and analyzed the morphological characteristics of acini with the method specialized for acinus structure to the micro-CT images of mouse lungs. The acinus morphology that we focused on was the cluster of acini belonging to the same terminal bronchiole, which is obtained by our multiscale-based segmentation algorithm. The volume and surface area of each acinus were measured by counting the voxels in the acinus and the triangular patches created on the wall surface of the acinus, respectively. A treestructure skeletonization algorithm was used for the analysis of the airway path in the acinus. Multiple clusters of isolated acini belonging to the same terminal bronchiole were obtained without floating voxels. The number of clusters was 3 and each cluster had 8, 9, and 9 acini. The volume and surface area of acinus were 0.125±0.09 mm3 (mean±SD.) and 10.09±6.70 mm3, respectively. The range of the volume of acinus was from 0.05 to 0.45 mm3 and the peak value was 0.1 mm3. In each cluster of acini, the maximum of acinar volume was found at the end of terminal bronchiole. The pathway lengths increased with the generation of airways. As for the average and the peak of acinar volume, the values reasonably agree well with the histology- based estimates of previous researches.

Original languageEnglish
Title of host publicationThe 15th International Conference on Biomedical Engineering, ICBME 2013
EditorsJames Goh
PublisherSpringer Verlag
Pages760-762
Number of pages3
ISBN (Electronic)9783319029122
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event15th International Conference on Biomedical Engineering, ICBME 2013 - Singapore, Singapore
Duration: Dec 4 2013Dec 7 2013

Publication series

NameIFMBE Proceedings
Volume43
ISSN (Print)1680-0737

Other

Other15th International Conference on Biomedical Engineering, ICBME 2013
CountrySingapore
CitySingapore
Period12/4/1312/7/13

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

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