Morphological and quantitative changes in mitochondria, plastids, and peroxisomes during the log-to-stationary transition of the growth phase in cultured tobacco BY-2 cells

Kiminori Toyooka, Mayuko Sato, Mayumi Wakazaki, Ken Matsuoka

Research output: Contribution to journalArticle

Abstract

ABSTRACT: We developed a wide-range and high-resolution transmission electron microscope acquisition system and obtained giga-pixel images of tobacco BY-2 cells during the log and stationary phases of cell growth. We demonstrated that the distribution and ultrastructure of compartments involved in membrane traffic (i.e., Golgi apparatus, multivesicular body, and vesicle cluster) change during the log-to-stationary transition. Mitochondria, peroxisomes, and plastids were also enumerated. Electron densities of mitochondria and peroxisomes were altered during the growth-phase shift, while their numbers were reduced by nearly half. Plastid structure dramatically changed from atypical to spherical with starch granules. Nearly the same number of plastids was observed in both log and stationary phases. These results indicate that mechanisms regulating organelle populations differ from organelle to organelle.

Original languageEnglish
Article numbere1149669
JournalPlant Signaling and Behavior
Volume11
Issue number3
DOIs
Publication statusPublished - Mar 3 2016

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peroxisomes
plastids
organelles
mitochondria
tobacco
transmission electron microscopes
cells
Golgi apparatus
starch granules
traffic
cell growth
ultrastructure
electrons

All Science Journal Classification (ASJC) codes

  • Plant Science

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Morphological and quantitative changes in mitochondria, plastids, and peroxisomes during the log-to-stationary transition of the growth phase in cultured tobacco BY-2 cells. / Toyooka, Kiminori; Sato, Mayuko; Wakazaki, Mayumi; Matsuoka, Ken.

In: Plant Signaling and Behavior, Vol. 11, No. 3, e1149669, 03.03.2016.

Research output: Contribution to journalArticle

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