Morphological changes in neurons by self-organized patterned films

Akinori Tsuruma, Masaru Tanaka, Nobuyuki Fukushima, Masatsugu Shimomura

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In tissue engineering, micro/nanofabrication is important to modify substrate surfaces for regulating the attachment and growth of cells. In neuroscience, it is significant for neural regeneration; this involves guiding and extending dendrites and axons by a cell culture scaffold which acts as an extra cellular matrix. In this study, we prepared highly regular porous honeycomb-patterned films by a simple casting technique and cultured neurons to investigate their morphologies on the patterned films. The morphologies of neurons were examined by a scanning electron microscope and a confocal laser scanning microscope. The neurons were round and the neurites extended randomly on the flat film. The patterns influenced the morphologies of neurons. The morphologies of neurons were changed by varying the pore size of the honeycomb- patterned films. The neurites spread along the rims of the honeycomb pattern. These results suggest that the self-organized honeycomb-patterned films are useful biomaterials for neural tissue engineering.

Original languageEnglish
Journale-Journal of Surface Science and Nanotechnology
Volume3
DOIs
Publication statusPublished - May 12 2005
Externally publishedYes

Fingerprint

neurons
Neurons
tissue engineering
Neurites
Tissue Engineering
Tissue engineering
neurology
Scanning
axons
scanning
nanofabrication
Biocompatible Materials
dendrites
Scaffolds (biology)
Neurosciences
Dendrites
rims
regeneration
Nanotechnology
Cell culture

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Condensed Matter Physics
  • Mechanics of Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

Cite this

Morphological changes in neurons by self-organized patterned films. / Tsuruma, Akinori; Tanaka, Masaru; Fukushima, Nobuyuki; Shimomura, Masatsugu.

In: e-Journal of Surface Science and Nanotechnology, Vol. 3, 12.05.2005.

Research output: Contribution to journalArticle

Tsuruma, Akinori ; Tanaka, Masaru ; Fukushima, Nobuyuki ; Shimomura, Masatsugu. / Morphological changes in neurons by self-organized patterned films. In: e-Journal of Surface Science and Nanotechnology. 2005 ; Vol. 3.
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